Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell lysate
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PPP1R2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
View Alternative Names
IPP2_HUMAN, MGC87148, PPP1R2 protein, Phosphatase inhibitor 2, Phosphoprotein phosphatase, Ppp1r2, Protein phosphatase 1 regulatory (inhibitor) subunit 2, Protein phosphatase 1 regulatory subunit 2, Protein phosphatase inhibitor 2
- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell lysate (AB259057)
Homozygous : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PPP1R2 plays a critical role in cellular regulation by modulating PP1 activity. It forms a complex with PP1 in which PPP1R2 binds directly to the catalytic subunit of PP1 inhibiting its phosphatase activity. By controlling PP1 activity PPP1R2 impacts processes like muscle contraction protein synthesis and neuronal signaling making it an active participant in maintaining cellular homeostasis.
Pathways
PPP1R2 functions within important signaling and dephosphorylation pathways. It is notably involved in the glycogen metabolism pathway where it regulates PP1 activity influencing glycogen synthesis and breakdown. Additionally it participates in the cell cycle regulation pathway interacting with other proteins such as PP1 to control cell proliferation and division. The regulation of these pathways by PPP1R2 highlights its importance in key cellular activities.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB265684
Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell line
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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