Human PPP1R3C knockout HeLa cell lysate
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PPP1R3C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.
View Alternative Names
OTTHUMP00000020089, PP1 subunit R5, PPP1R5, PPR3C_HUMAN, PTG, Phosphatase 1, regulatory inhibitor subunit 5, Protein phosphatase 1 inhibitor 3C, Protein phosphatase 1 regulatory subunit 3C, Protein phosphatase 1 regulatory subunit 5, Protein targeting to glycogen, R5, protein phosphatase 1, regulatory (inhibitor) subunit 3C
- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R3C knockout HeLa cell lysate (AB259058)
Homozygous : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PPP1R3C protein binds to glycogen and PP1 forming a complex with these substrates. This interaction plays a role in the regulation of glucose homeostasis by controlling glycogen synthesis and breakdown. Such processes are important for energy storage and release in muscle and liver tissues. By integrating within this complex PPP1R3C influences the activity and localization of PP1 facilitating efficient glycogen regulation.
Pathways
PPP1R3C participates in critical pathways like glycogen metabolism and insulin signaling. The protein is closely associated with key metabolic proteins such as glycogen synthase. Within these pathways PPP1R3C modifies the enzymatic activity of its pathway partners to balance glucose levels in response to insulin. This involvement supports metabolic flexibility and energy management in response to dietary changes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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