Human PPP2R1B knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
PPP2R1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp insertion in exon 1.
View Alternative Names
2AAB_HUMAN, PP2A A beta, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PP2A, subunit A, PR65 beta isoform, PP2A, subunit A, R1 beta isoform, PPP4R1, PR65B, Protein phosphatase 2, regulatory subunit A, beta, Protein phosphatase 2, structural/regulatory subunit A, beta, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, beta isoform of regulatory subunit A, protein phosphatase 2, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform, protein phosphatase 2, structural/regulatory subunit A, beta isoform
- WB
Lab
Western blot - Human PPP2R1B knockout HEK-293T cell lysate (AB258133)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : PPP2R1B knockout HEK293T cell lysate (20 ug)
Lane 3 : Caco-2 cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)
ab154815 was shown to specifically react with PPP2R1B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266729 (knockout cell lysate ab258133) was used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PPP2R1B antibody [EPR10158] (<a href='/en-us/products/primary-antibodies/ppp2r1b-antibody-epr10158-ab154815'>ab154815</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
PPP2R1B knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human PPP2R1B knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ppp2r1b-knockout-hek-293t-cell-line-ab266729'>ab266729</a>)
Lane 3:
Caco-2 cell lysate at 20 µg
Lane 4:
HeLa cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PPP2R1B knockout HEK-293T cell lysate (AB258133)
Homozygous : 37 bp insertion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PPP2R1B integrates into the PP2A holoenzyme complex a major serine/threonine phosphatase. This complex regulates numerous cellular processes including cell growth division and signal transduction. PPP2R1B plays a role in maintaining cellular homeostasis through its regulatory activities. By doing so it links to various signaling events some of which control oncogenes and tumor suppressors demonstrating the protein's influence over cell cycle regulation and apoptosis.
Pathways
PPP2R1B plays a significant role in the Raf/MEK/ERK signaling pathway and the PI3K/AKT pathway. Through these pathways PPP2R1B interacts with PDPK1 and AKT1 modulating their phosphorylation status and thereby affecting cell proliferation and survival. By influencing such pathways PPP2R1B helps determine cellular responses to external stimuli impacting processes like differentiation and metabolism that are vital for organismal function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)](https://content.abcam.com/products/images/ppp2r1b-antibody-epr10158-ab154815--western-blot-img57473.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com