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PPP2R1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp insertion in exon 1.

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Images

Western blot - Human PPP2R1B knockout HEK-293T cell lysate (AB258133), expandable thumbnail
  • Sanger Sequencing - Human PPP2R1B knockout HEK-293T cell lysate (AB258133), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp insertion in exon 1.

Alternative names

2AAB_HUMAN, PP2A A beta, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PP2A, subunit A, PR65 beta isoform, PP2A, subunit A, R1 beta isoform, PPP4R1, PR65B, Protein phosphatase 2, regulatory subunit A, beta, Protein phosphatase 2, structural/regulatory subunit A, beta, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, beta isoform of regulatory subunit A, protein phosphatase 2, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform, protein phosphatase 2, structural/regulatory subunit A, beta isoform

What's included?

1 Kit
Components
Human PPP2R1B knockout HEK293T cell lysate
1 x 100 µg
Human wild-type HEK293T cell lysate
1 x 100 µg

Recommended products

PPP2R1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp insertion in exon 1.

Key facts

Cell type
HEK-293T
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp insertion in exon 1.
Concentration
Loading...

Properties

Gene name
PPP2R1B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein PPP2R1B also known as Protein Phosphatase 2 Scaffold Subunit A beta isoform acts as a scaffold for the assembly of PP2A complexes. PPP2R1B has a molecular mass of approximately 66 kDa. The protein expresses widely across various tissues with notable presence in the brain heart and skeletal muscle. As a scaffold subunit PPP2R1B coordinates the structural integrity necessary for phosphatase activity by recruiting regulatory and catalytic subunits important to its function.

Biological function summary

PPP2R1B integrates into the PP2A holoenzyme complex a major serine/threonine phosphatase. This complex regulates numerous cellular processes including cell growth division and signal transduction. PPP2R1B plays a role in maintaining cellular homeostasis through its regulatory activities. By doing so it links to various signaling events some of which control oncogenes and tumor suppressors demonstrating the protein's influence over cell cycle regulation and apoptosis.

Pathways

PPP2R1B plays a significant role in the Raf/MEK/ERK signaling pathway and the PI3K/AKT pathway. Through these pathways PPP2R1B interacts with PDPK1 and AKT1 modulating their phosphorylation status and thereby affecting cell proliferation and survival. By influencing such pathways PPP2R1B helps determine cellular responses to external stimuli impacting processes like differentiation and metabolism that are vital for organismal function.

Associated diseases and disorders

PPP2R1B links to cancer and Alzheimer's disease. Mutations or downregulation in PPP2R1B have been implicated in colorectal cancer where it alters the PP2A activity and allows unregulated cellular proliferation. In Alzheimer's disease PPP2R1B's interaction with tau protein goes awry contributing to neurofibrillary tangle formation. These connections highlight the importance of PPP2R1B in understanding the molecular basis of these diseases and potential therapeutic targets.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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