Human PPP6R1 (SAPS1) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PPP6R1 (SAPS1) knockout HEK-293T cell lysate (AB259059)
Allele-1 : Insertion of the selection cassette in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human PPP6R1 (SAPS1) knockout HEK-293T cell lysate (AB259059)
Allele-3 : Insertion of the selection cassette in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human PPP6R1 (SAPS1) knockout HEK-293T cell lysate (AB259059)
Allele-2 : 1 bp deletion in exon5
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SAPS1 interacts with the PP6 catalytic subunit forming the PP6 complex which is involved in cell cycle regulation and DNA repair. This complex regulates various cellular functions by dephosphorylation of specific target proteins. SAPS1 ensures proper DNA repair mechanisms and checkpoint controls during cell division essential for maintaining genomic stability. This role positions SAPS1 as a pivotal player in the cellular stress response.
Pathways
SAPS1 influences important signaling pathways including the NF-kB signaling and SAPK/JNK pathways. In these pathways SAPS1 helps regulate inflammatory responses and stress-activated protein kinases through its interaction with the PP6 complex. It relates closely to proteins like IkB as part of NF-kB signaling where SAPS1 through PP6 dephosphorylates components involved in the pathway to modulate its activity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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