Human PRCC (Papillary renal cell carcinoma) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PRCC (Papillary renal cell carcinoma) knockout HEK-293T cell lysate (AB259061)
Allele-1 : 13 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human PRCC (Papillary renal cell carcinoma) knockout HEK-293T cell lysate (AB259061)
Allele-2 : 186 bp deletion in exon1
Complementary Products
Cell Lines & Lysates
AB266268
Human PRCC (Papillary renal cell carcinoma) knockout HEK-293T cell line
- 0
- 0
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PRCC forms as a type of cancer characterized by the formation of finger-like projections in the kidney. It does not align to a single protein or simple biological function but involves components like alterations in the MET pathway an independent complex soliciting increased proliferative and migratory signals within renal tissues. This process can lead to the unusual cell growth that distinguishes PRCC in affected kidneys.
Pathways
MET signaling pathway and the associated HGF (hepatocyte growth factor) signaling cascade are closely involved in papillary renal phenotypes. These pathways contribute significantly to cell growth migration and differentiation regulation within the renal system. It's related to proteins such as RAS and PI3K which play essential roles in further amplifying cellular signals that contribute to oncogenesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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