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AB257040

Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate

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PRDX1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.

View Alternative Names

Heme binding 23 kDa protein, MSP23, NKEF-A, Natural killer cell-enhancing factor A, OSF3, Osteoblast specific factor 3, PAGB, PRDX1_HUMAN, PRX1, PagA, Peroxiredoxin-1, Proliferation associated gene A, Proliferation-associated gene protein, PrxI, TDPX2, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2

3 Images
Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (AB257040)
  • WB

Unknown

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (AB257040)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : PRDX1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)

ab109506 was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-1-pag-antibody-epr5434-ab109506'>ab109506</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

PRDX1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-prdx1-peroxiredoxin-1-pag-knockout-hek-293t-cell-line-ab266842'>ab266842</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 26 kDa

false

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (AB257040)
  • WB

Lab

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (AB257040)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : PRDX1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)

ab109498 was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-1-pag-antibody-epr5433-ab109498'>ab109498</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

PRDX1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-prdx1-peroxiredoxin-1-pag-knockout-hek-293t-cell-line-ab266842'>ab266842</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 22 kDa,41 kDa,45 kDa,78 kDa

Observed band size: 25 kDa,26 kDa,47 kDa,49 kDa,78 kDa

false

Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (AB257040)
  • Sanger seq

Unknown

Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (AB257040)

Homozygous : 1 bp deletion in exon2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PRDX1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.
Biological function summary

Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.

Pathways

Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.

Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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