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AB257041

Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate

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PRDX2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2.

View Alternative Names

Epididymis secretory sperm binding protein Li 2a, HEL S 2a, MGC4104, NKEF-B, Natural Killer Enhancing Factor B, Natural killer cell-enhancing factor B, PRDX2_HUMAN, PRX2, PRXII, PTX 1, Peroxiredoxin-2, PrP, TDPX1, TPX1, TSA, Thiol Specific Antioxidant 1, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Torin

3 Images
Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (AB257041)
  • WB

Lab

Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (AB257041)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : PRDX2 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab109367 observed at 22 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109367 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] was shown to specifically react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266392 (knockout cell lysate ab257041) was used. Wild-type and Peroxiredoxin 2/PRP knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-2-prp-antibody-epr5154-ab109367'>ab109367</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PRDX2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-prdx2-peroxiredoxin-2-prp-knockout-hek-293t-cell-line-ab266392'>ab266392</a>)

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (AB257041)
  • WB

Lab

Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (AB257041)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : PRDX2 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab133481 observed at 22 kDa. Red - loading control ab8245 observed at 37 kDa.

ab133481 Anti-Peroxiredoxin 2/PRP antibody [EPR5155] was shown to specifically react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266392 (knockout cell lysate ab257041) was used. Wild-type and Peroxiredoxin 2/PRP knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133481 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-2-prp-antibody-epr5155-ab133481'>ab133481</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PRDX2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-prdx2-peroxiredoxin-2-prp-knockout-hek-293t-cell-line-ab266392'>ab266392</a>)

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Sanger Sequencing - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (AB257041)
  • Sanger seq

Unknown

Sanger Sequencing - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (AB257041)

Homozygous : 2 bp deletion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PRDX2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 2 (also known as PRP or PRDX2) is an antioxidant enzyme that plays an important role in reducing peroxides and protecting cells from oxidative damage. The target consisting of approximately 22kDa mass is expressed heavily in erythrocytes but can also be found in other tissues like the heart and liver. PRDX2 belongs to the peroxiredoxin family and its redox activity contributes significantly to cellular homeostasis and defense against oxidative stress.
Biological function summary

Peroxiredoxins like PRDX2 function by breaking down hydrogen peroxide and organic hydroperoxides safeguarding cells from oxidative harm. PRDX2 often forms homodimers or higher-order oligomers which influence its catalytic efficiency and chaperone activity. Within the cell it not only acts independently but also associates with other cellular components reflecting its participation in important cellular processes including cell proliferation and apoptosis.

Pathways

PRDX2 engages significantly within antioxidant defense systems specifically the thioredoxin pathway. It interacts with thioredoxin reductase and thioredoxin to facilitate the reduction of peroxides maintaining the cell’s redox balance. Furthermore PRDX2 intersects with cellular signaling pathways associated with inflammation and the regulation of cell death where proteins like NRF2 and KEAP1 play important roles in managing oxidant-inducible gene expression.

Disruptions in PRDX2 function connect to conditions like cancer and cardiovascular diseases. In cancer altered peroxiredoxin 2 expression correlates to tumor progression and resistance to chemotherapy implicating its interaction with proteins like NF-κB in enhancing cell survival. In cardiovascular diseases oxidative stress mediated by PRDX2 imbalance can lead to myocardial infarction and heart failure linking it to proteins such as SOD2 involved in mitochondrial defense.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB266392

Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line

Advanced Validation

Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (AB266392)

  • 0
  • 0
Cell Lines & Lysates

AB257343

Human ADK knockout HeLa cell lysate

Western blot - Human ADK knockout HeLa cell lysate (AB257343)

  • 0
  • 0
Primary Antibodies

AB109367

Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB109367)

  • 5
  • 2
Assay Kits

AB277456

Human Peroxiredoxin 2/PRP ELISA Kit

ELISA - Human Peroxiredoxin 2/PRP ELISA Kit (AB277456)

  • 0
  • 0

Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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