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AB257264

Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell lysate

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PRDX4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

View Alternative Names

AOE37-2, Antioxidant enzyme 372, Antioxidant enzyme AOE372, EC 1.11.1.15, PRDX4_HUMAN, PRX 4, Peroxiredoxin IV, Peroxiredoxin-4, Prx-IV, TRANK, Thioredoxin Peroxidase (Antioxidant Enzyme), Thioredoxin peroxidase, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372

2 Images
Western blot - Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell lysate (AB257264)
  • WB

Lab

Western blot - Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell lysate (AB257264)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : PRDX4 knockout HEK293T cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)

ab184167 was shown to specifically react with Peroxiredoxin 4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266727 (knockout cell lysate ab257264) was used. Wild-type and Peroxiredoxin 4 knockout samples were subjected to SDS-PAGE. ab184167 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-4-antibody-epr15458b-ab184167'>ab184167</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

PRDX4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-prdx4-peroxiredoxin-4-knockout-hek-293t-cell-line-ab266727'>ab266727</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 177 kDa,189 kDa,31 kDa

Observed band size: 27 kDa

false

Sanger Sequencing - Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell lysate (AB257264)
  • Sanger seq

Unknown

Sanger Sequencing - Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell lysate (AB257264)

Homozygous : Insertion of the selection cassette in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PRDX4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 4 also known as Prdx4 is a protein that acts as an antioxidant enzyme and belongs to the peroxiredoxin family. It influences the reduction of hydrogen peroxide and organic hydroperoxides. The molecular mass of Prdx4 is approximately 31 kDa. It is expressed mainly in the endoplasmic reticulum but you can find some expression in the cytosol as well.
Biological function summary

Prdx4 plays a list of roles in cellular defense against oxidative stress. It not only reduces peroxides but also contributes to the maintenance of protein folding by modifying disulfide bonds in the endoplasmic reticulum. Prdx4 does not function as part of a larger complex but acts independently within its compartments. The protective functions of Prdx4 are important for cell survival and proper cellular function.

Pathways

Prdx4 interacts with oxidative stress response pathways. Specifically it participates in the redox signaling pathways that regulate cellular reactive oxygen species levels. Peroxiredoxin 4 is closely associated with proteins such as thioredoxin in these pathways. Together they regulate the cellular redox environment which is important for maintaining cellular homeostasis.

Oxidative stress imbalance implicates Prdx4 in conditions like diabetes and cancer. For example in diabetes altered Prdx4 levels disturb normal cell function due to increased oxidative stress. In cancer Prdx4 can influence tumor progression by modulating the antioxidant capacity within cells. Prdx4 also shows a functional connection to proteins like glutathione peroxidase in regulating oxidative stress-related pathologies.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB184167

Anti-Peroxiredoxin 4 antibody [EPR15458(B)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (AB184167)

  • 5
  • 1
Cell Lines & Lysates

AB266727

Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell line

Advanced Validation

Western blot - Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell line (AB266727)

  • 0
  • 0
Primary Antibodies

AB316329

Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

  • 0
  • 0
Primary Antibodies

AB129097

Anti-FKBP52 antibody [EPR6618]

RabMAb|Recombinant

Western blot - Anti-FKBP52 antibody [EPR6618] (AB129097)

  • 0
  • 0

Product promise

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