Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell lysate
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PRKAR2A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp deletion in exon 1.
View Alternative Names
KAP2_HUMAN, MGC3606, PRKA R2, PRKAR 2, PRKAR2, PRKAR2A, Protein kinase A RII alpha subunit, Protein kinase cAMP dependent regulatory type II alpha, cAMP dependent protein kinase regulatory subunit RII alpha, cAMP dependent protein kinase regulatory subunit alpha 2, cAMP dependent protein kinase type II alpha regulatory chain, cAMP-dependent protein kinase type II-alpha regulatory subunit
- WB
Lab
Western blot - Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell lysate (AB257607)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : PRKAR2A knockout HeLa cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)
ab32514 was shown to specifically react with PKA R2/PKR2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265748 (knockout cell lysate ab257607) was used. Wild-type and PKA R2/PKR2 knockout samples were subjected to SDS-PAGE. ab32514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PKA R2/PKR2 antibody [Y116] (<a href='/en-us/products/primary-antibodies/pka-r2-pkr2-antibody-y116-ab32514'>ab32514</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PRKAR2A knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-prkar2a-pka-r2-pkr2-knockout-hela-cell-line-ab265748'>ab265748</a>)
Lane 3:
K-562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell lysate (AB257607)
Allele-1 : 4 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PRKAR2A (PKA R2/PKR2) knockout HeLa cell lysate (AB257607)
Allele-2 : 1 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PKR2 regulates the activity of PKA by binding to the catalytic subunit in the absence of cAMP therefore inhibiting its activity. When cAMP is present it binds to the regulatory subunit PKR2 leading to the release and activation of the catalytic subunit of PKA. PKR2 is an integral part of the PKA holoenzyme complex which plays a critical role in mediating cellular responses to hormonal stimulation.
Pathways
PKR2 is heavily involved in the cAMP-dependent signaling pathway. This pathway is essential for translating extracellular signals into cellular actions. It interacts with various effector proteins including adenylate cyclases and phosphodiesterases to modulate responses such as gene transcription metabolism and memory formation. PKR2-related activity influences pathways shared with proteins like the G-protein subunits which facilitate the effects of neurotransmitters.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB32514
Anti-PKA R2/PKR2 antibody [Y116]
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Flow Cytometry (Intracellular) - Anti-PKA R2/PKR2 antibody [Y116] (AB32514)](https://content.abcam.com/products/images/pka-r2-pkr2-antibody-y116-ab32514--flow-cytometry-intracellular-img27641.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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