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AB280102

Human PSD95 knockout SH-SY5Y cell lysate

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DLG4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included.

View Alternative Names

DLG4_HUMAN, Discs large homolog 4, Disks large homolog 4, FLJ97752, FLJ98574, Human post synaptic density protein 95, PSD 95, Post synaptic density protein 95, SAP-90, Synapse-associated protein 90, Tax interaction protein 15

2 Images
Western blot - Human PSD95 knockout SH-SY5Y cell lysate (AB280102)
  • WB

Lab

Western blot - Human PSD95 knockout SH-SY5Y cell lysate (AB280102)

Lane 1 : Wild-type SH-SY5Y cell lysate 20 μg
Lane 2 : DLG4 knockout SH-SY5Y cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : HL-60 cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab238135 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab238135 was shown to react with PSD95 in wild-type SH-SY5Y cells in Western blot with loss of signal observed in DLG4 knockout cell line ab280043 (DLG4 knockout cell lysate ab280102). Wild-type SH-SY5Y and DLG4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab238135 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (<a href='/en-us/products/primary-antibodies/psd95-antibody-epr23124-118-synaptic-marker-ab238135'>ab238135</a>) at 1/2000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

DLG4 knockout SH-SY5Y cell lysate at 20 µg

Lane 2:

Western blot - Human PSD95 knockout SH-SY5Y cell line (<a href='/en-us/products/cell-lines/human-psd95-knockout-sh-sy5y-cell-line-ab280043'>ab280043</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HL-60 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 80 kDa

Observed band size: 75 kDa

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Sanger Sequencing - Human PSD95 knockout SH-SY5Y cell lysate (AB280102)
  • Sanger seq

Supplier Data

Sanger Sequencing - Human PSD95 knockout SH-SY5Y cell lysate (AB280102)

Human PSD95 KO in SH-SY5Y Cells with 40 bp Deletion in Exon 5

Key facts

Cell type

SH-SY5Y

Species or organism

Human

Tissue

Bone marrow

Knockout validation

Sanger Sequencing,Western blot

Disease

Neuroblastoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
DLG4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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