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AB273772

Human PSENEN (PEN2) knockout HEK-293 cell lysate

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PSENEN KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 1 bp insertion; Frameshift: 100%.

View Alternative Names

Gamma Secretase Subunit PEN2, Gamma-secretase subunit PEN-2, Hematopoietic stem/progenitor cells protein MDS033, MDS033, MSTP064, PEN2_HUMAN, PSEN2, Presenilin Enhancer 2, Presenilin enhancer 2 homolog, Presenilin enhancer protein 2, psenen

2 Images
Western blot - Human PSENEN (PEN2) knockout HEK-293 cell lysate (AB273772)
  • WB

Lab

Western blot - Human PSENEN (PEN2) knockout HEK-293 cell lysate (AB273772)

Lane 1 : Wild-type HEK-293 cell lysate 20 μg

Lane 2 : PSENEN knockout HEK-293 cell lysate 20 μg

Lane 3 : Neuro-2a cell lysate 20 μg

Lane 4 : Daudi cell lysate 20 μg

False colour image of Western blot : Anti-PEN2 antibody [EPR9200] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154830 was shown to bind specifically to PEN2. A band was observed at 12 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in PSENEN knockout cell line ab273720 (knockout cell lysate ab273772). To generate this image, wild-type and PSENEN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-PEN2 antibody [EPR9200] (<a href='/en-us/products/primary-antibodies/pen2-antibody-epr9200-ab154830'>ab154830</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 cell lysate at 20 µg

Lane 2:

PSENEN knockout HEK-293 cell lysate at 20 µg

Lane 2:

Western blot - Human PSENEN (PEN2) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-psenen-pen2-knockout-hek-293-cell-line-ab273720'>ab273720</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 12 kDa

Observed band size: 12 kDa

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Next Generation Sequencing - Human PSENEN (PEN2) knockout HEK-293 cell lysate (AB273772)
  • NGS

Supplier Data

Next Generation Sequencing - Human PSENEN (PEN2) knockout HEK-293 cell lysate (AB273772)

Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 1 bp insertion; Frameshift : 100%

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 1 bp insertion; Frameshift: 100%

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PSENEN
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEN2 also known as Presenilin Enhancer 2 is a component of the gamma-secretase complex. It has a molecular mass of approximately 12 kDa. PEN2 is expressed in a variety of tissues including neuronal cells and across several cell lines like the HEK 293. This protein plays an essential mechanical role in stabilizing and activating the gamma-secretase complex which is necessary for its protease activity.
Biological function summary

PEN2 is an important element of the gamma-secretase complex composed of presenilin nicastrin APH-1 and PEN2. Its role involves the facilitation of proper maturation and assembly of the complex contributing to the proteolytic processing of several type I transmembrane proteins including the amyloid precursor protein (APP) and Notch. PEN2 plays a part in these cellular processes by ensuring that the gamma-secretase complex functions correctly impacting cellular signaling and communication.

Pathways

Gamma-secretase activity where PEN2 is involved is central to the Notch signaling pathway and the amyloidogenic pathway. The Notch signaling pathway is fundamental in cell differentiation proliferation and apoptosis and relies significantly on the function of the gamma-secretase complex. The amyloidogenic pathway involves the processing of APP to produce amyloid-beta peptides which PEN2's role in the gamma-secretase complex influences. Related proteins in these pathways include presenilin which is an active component in Notch and APP cleavage.

Mutations or dysregulation of PEN2 can have links to Alzheimer's disease and certain cancers. Alzheimer's disease is associated with the production of amyloid-beta peptides which accumulate into plaques a process influenced by PEN2 through gamma-secretase activity with APP. Dysregulated Notch signaling where PEN2 is involved is linked to various cancers due to its role in controlling cell fate decisions. PEN2's interactions with proteins like presenilin and APP highlight its potential impact on these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB154830

Anti-PEN2 antibody [EPR9200]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (AB154830)

  • 1
  • 3
Proteins & Peptides

AB289755

Recombinant Human PEN2 protein

SDS-PAGE - Recombinant Human PEN2 protein (AB289755)

  • 0
  • 0
Cell Lines & Lysates

AB273720

Human PSENEN (PEN2) knockout HEK-293 cell line

Advanced Validation

Western blot - Human PSENEN (PEN2) knockout HEK-293 cell line (AB273720)

  • 0
  • 0

Product promise

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