Human PSMB10 (MECL1) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
PSMB10 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
View Alternative Names
FLJ00366, LMP10, Low molecular mass protein 10, MECL1, MGC1665, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, OTTHUMP00000174858, PSB10_HUMAN, PSMB 10, Proteasome (prosome macropain) subunit beta type 10, Proteasome MECl-1, Proteasome beta 10 subunit, Proteasome catalytic subunit 2i, Proteasome subunit MECL1, Proteasome subunit beta 7i, Proteasome subunit beta type-10, Proteasome subunit beta-2i, beta2i
- WB
Lab
Western blot - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : PSMB10 knockout HeLa cell lysate (20 ug)
Lane 3 : Daudi cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)
ab183506 was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used. Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE. ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (<a href='/en-us/products/primary-antibodies/psmb10-mecl1-antibody-epr14902-ab183506'>ab183506</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PSMB10 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PSMB10 (MECL1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-psmb10-mecl1-knockout-hela-cell-line-ab264923'>ab264923</a>)
Lane 3:
Daudi cell lysate at 20 µg
Lane 4:
Raji cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)
Homozygous : 1 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PSMB10/MECL1 plays a significant role in immune responses. It forms part of the immunoproteasome a variant of the proteasome complex which enhances the generation of antigenic peptides presented on MHC class I molecules. This function is essential for effective immune surveillance and initializing adaptive immunity. In particular PSMB10's influence on antigen processing positions it as an important player in modulating immune system activity.
Pathways
PSMB10/MECL1 participates in pathways associated with protein catabolism and immune signaling. It is involved in the ubiquitin-proteasome pathway which is critical for maintaining cellular protein balance. The function of PSMB10 within the immunoproteasome links it to immune signaling pathways. It has associations with other 20S proteasome subunits such as PSMB8 and PSMB9 which also contribute to the formation of the immunoproteasome complex.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB183506
Anti-PSMB10/MECL1 antibody [EPR14902]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img5033.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com