Human PSMD8 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PSMD8 knockout HEK-293T cell lysate (AB258609)
Allele-1 : 22 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PSMD8 knockout HEK-293T cell lysate (AB258609)
Allele-2 : 19 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PSMD8 contributes to the assembly and function of the 26S proteasome which is a multi-subunit complex responsible for degrading ubiquitinated proteins. It acts as a part of the regulatory particle enabling substrate recognition and unfolding. Through its function PSMD8 is involved in the ubiquitin-proteasome system which is key in cellular regulation mechanisms such as cell cycle control apoptosis and DNA repair. This highlights its importance in maintaining cell function and integrity.
Pathways
Protein homeostasis and degradation represent important pathways in which PSMD8 participates. It plays a role in the ubiquitin-proteasome pathway interacting with proteins within this system like Rpn11 and Rpt5 which are also parts of the proteasome complex. Additionally the involvement of PSMD8 in the protein degradation pathway links it to the regulation of transcription factors influencing various signaling pathways that control cell proliferation and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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