PSME1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.
Alternative names
11S regulator complex alpha subunit, 11S regulator complex subunit alpha, 29 kD MCP activator subunit, Activator of multicatalytic protease subunit 1, IFI5111, IGUP I-5111, Interferon gamma IEF SSP 5111, Interferon gamma inducible protein 5111, Interferon gamma up-regulated I-5111 protein, MGC8628, PA28a, PA28alpha, PSME1_HUMAN, Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), Proteasome activator 28 subunit alpha, Proteasome activator complex subunit 1, Proteasome activator subunit 1, REG-alpha
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PSME1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.
- Concentration
Properties
- Gene name
- PSME1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSME1 also known as PA28α (proteasome activator complex subunit 1) is a protein that functions as a regulatory component of the 20S proteasome. It has a molecular weight of approximately 28 kDa. This protein is expressed in various tissues with noticeable expression in immune-related tissues such as the spleen and thymus. PSME1 binds to proteasome core particles and modifies their activity by opening access to their catalytic core honing the degradation of ubiquitinated proteins.
- Biological function summary
PSME1 modulates protein degradation processes and participates as a component of the immunoproteasome. The immunoproteasome is a complex that processes peptides for presentation on MHC class I molecules vital for immune response. This involvement transforms PSME1 into an important player in antigen processing and presentation influencing T-cell activation and immune surveillance. PSME1 affects immune system adaptability and efficiency impacting infection response and immune regulation.
- Pathways
PSME1 is a significant participant in the ubiquitin-proteasome system and the MHC class I antigen processing pathway. It directly interacts with other proteasome components like the catalytic 20S core and the 11S regulator. The interplay with these proteins steers the precision and output of antigenic peptides essential for T-cell mediated immune recognition. By influencing these pathways PSME1 links the protein degradation machinery to adaptive immunity.
- Associated diseases and disorders
PSME1 has connections to autoimmune diseases and cancers. Alterations in its function or expression can disrupt regular proteasome activity leading to improper immune responses such as in autoimmune pathologies like systemic lupus erythematosus. Furthermore PSME1's role in antigen processing intersects with other proteins such as TAP1 affecting immune evasion strategies seen in certain cancers. These interactions suggest that PSME1 is a contributor in both immune-related health issues and cancer progression.
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2 product images
Western blot - Human PSME1 knockout HEK-293T cell lysate (ab257613)
Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2:PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 3:MCF7 (Human breast adenocarcinoma cell line) whole cell lysate (20 ug)
Lane 4:Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (20 ug)Anti-PSME1 antibody [EPR10967(B)] ab155091 was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human PSME1 knockout HEK-293T cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. Anti-PSME1 antibody [EPR10967(B)] ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PSME1 antibody [EPR10967(B)] (Anti-PSME1 antibody [EPR10967(B)] ab155091) at 1/1000 dilution
Lane 1: Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: Western blot - Human PSME1 knockout HEK-293T cell line (Human PSME1 knockout HEK-293T cell line ab266192)
Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Sanger Sequencing - Human PSME1 knockout HEK-293T cell lysate (ab257613)
Homozygous: 14 bp deletion in exon 3
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