JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258148

Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

PSME4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2.

View Alternative Names

KIAA0077, PA200, PSME4_HUMAN, Proteasome Activator 200 kDa, Proteasome activator PA200, Proteasome activator complex subunit 4

2 Images
Sanger Sequencing - Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell lysate (AB258148)
  • Sanger seq

Unknown

Sanger Sequencing - Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell lysate (AB258148)

Allele-2 : 1 bp insertion in exon2

Sanger Sequencing - Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell lysate (AB258148)
  • Sanger seq

Unknown

Sanger Sequencing - Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell lysate (AB258148)

Allele-1 : 2 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2.

Disease

Adenocarcinoma

style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258148-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PSME4 knockout HeLa cell lysate", "number":"AB258148-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258148-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PSME4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome Activator Subunit 4 (PSME4) also known as PA200 is an important component in regulating protein degradation. It belongs to the family of proteasome activator subunits and has a molecular mass of approximately 230 kDa. PSME4 is mainly expressed in the nucleus of cells and modulates proteasome activity by binding to the proteasome core particle. Through facilitating protein breakdown PSME4 significantly influences various cellular processes including the elimination of oxidatively damaged proteins.
Biological function summary

PSME4 functions as part of a complex that enhances proteasome-mediated protein degradation. This complex formation improves the efficiency of protein processing by recruiting specific substrates for degradation. PSME4 is involved in DNA repair processes and plays a role in spermatogenesis contributing to proper cell cycle progression and genomic stability. The protein's activity in these biological functions demonstrates its importance in maintaining cellular homeostasis.

Pathways

PSME4 is intricately involved in the ubiquitin-proteasome pathway and the DNA damage response pathway which are essential for maintaining protein quality control and genomic integrity. PSME4 interacts with other proteins such as RAD23B facilitating the repair of damaged DNA. By integrating into these pathways PSME4 ensures the proper execution of critical cellular processes and responds to stress-related signals.

Alterations in PSME4 expression or function have connections to cancer progression and neurodegenerative diseases. In cancer PSME4 may influence tumor growth and resistance to therapy through its role in DNA repair and protein turnover. Additionally in neurodegenerative conditions like Alzheimer's disease PSME4-associated proteasome dysfunction contributes to the accumulation of toxic protein aggregates. Understanding PSME4 interactions with proteins like p53 in cancer or tau in neurodegeneration offers insights into potential therapeutic targets.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB181203

Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal

RabMAb|Recombinant

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (AB181203)

  • 0
  • 0
Cell Lines & Lysates

AB265661

Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell line

Sanger Sequencing - Human PSME4 (Proteasome Activator Subunit 4) knockout HeLa cell line (AB265661)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com