Human PSRC1 (DDA3) knockout HeLa cell lysate
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PSRC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.
View Alternative Names
DDA3, DDA3, mouse, homolog of, Differential display and activated by p53, FP3214, OTTHUMP00000013785, OTTHUMP00000013786, PSRC1_HUMAN, Proline/serine-rich coiled-coil 1, Proline/serine-rich coiled-coil protein 1, p53-regulated DDA3
- Sanger seq
Unknown
Sanger Sequencing - Human PSRC1 (DDA3) knockout HeLa cell lysate (AB263317)
Homozygous : Insertion of the selection cassette in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PSRC1 plays an important role in ensuring correct chromosome segregation during cell division. It functions as part of the mitotic spindle assembly complex and is responsible for promoting stable attachment of microtubules to kinetochores which is critical for accurate chromosomal alignment. These functions involve interactions with proteins related to spindle formation and checkpoint signaling.
Pathways
PSRC1 acts in the mitotic spindle checkpoint pathway and the microtubule dynamics pathway. Its interaction with proteins like CLASP1 and APC important for microtubule stability and dynamics highlights its contribution to maintaining proper mitotic progression. PSRC1 ensures successful completion of mitosis by coordinating these pathways important for cell division fidelity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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