Human PTBP1 knockout HeLa cell lysate
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PTBP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
View Alternative Names
57 kDa RNA-binding protein PPTB-1, HNRNP-I, HNRPI, Heterogeneous Nuclear Ribonucleoprotein Polypeptide I, Heterogeneous nuclear ribonucleoprotein I, MGC10830, MGC8461, PTB, PTB 1, PTB 2, PTB 3, PTB 4, PTB T, PTBP1_HUMAN, Polypyrimidine tract binding protein, Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), Polypyrimidine tract-binding protein 1, RNA Binding Protein, pPTB
- WB
Lab
Western blot - Human PTBP1 knockout HeLa cell lysate (AB257614)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : PTBP1 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133734 Anti-PTBP1 antibody [EPR9048(B)] was shown to specifically react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PTBP1 antibody [EPR9048(B)] (<a href='/en-us/products/primary-antibodies/ptbp1-antibody-epr9048b-ab133734'>ab133734</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PTBP1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PTBP1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ptbp1-knockout-hela-cell-line-ab265155'>ab265155</a>)
Predicted band size: 57 kDa
Observed band size: 57 kDa
false
- WB
Lab
Western blot - Human PTBP1 knockout HeLa cell lysate (AB257614)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : PTBP1 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - loading control ab8245 observed at 37 kDa.
ab134950 Anti-PTBP1 antibody [EPR9049(B)] was shown to specifically react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134950 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PTBP1 antibody [EPR9049(B)] (<a href='/en-us/products/primary-antibodies/ptbp1-antibody-epr9049b-ab134950'>ab134950</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PTBP1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PTBP1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ptbp1-knockout-hela-cell-line-ab265155'>ab265155</a>)
Predicted band size: 57 kDa
Observed band size: 57 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PTBP1 knockout HeLa cell lysate (AB257614)
Homozygous : 1 bp insertion in exon4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PTBP1 influences the stability transport and translation of mRNA molecules. It is part of the heterogeneous nuclear ribonucleoprotein complex which plays a fundamental role in the processing and modification of pre-mRNA into mature mRNA. This activity impacts the expression of a wide range of genes affecting cellular function and growth. PTBP1 regulates mRNA metabolism contributing significantly to post-transcriptional gene regulation.
Pathways
The involvement of PTBP1 spans the management of alternative splicing and the modulation of gene expression. It participates importantly in the alternative splicing pathway alongside proteins like PTBP2 which perform overlapping functions in neurons. PTBP1 affects exon inclusion or exclusion influencing the genetic diversity presented in protein forms within cells. It also plays a role in the exon junction complex pathway impacting post-translational modifications and protein interactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] (AB133734)](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-ab133734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83877.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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