Human PTGR1 knockout HeLa cell lysate
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PTGR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
15-oxoprostaglandin 13-reductase, FLJ99229, LTB4DH, MGC34943, NADP-dependent leukotriene B4 12-hydroxydehydrogenase, PGR1, PRG-1, PTGR1_HUMAN, Prostaglandin reductase 1, RP11-16L21.1, ZADH3, leukotriene B4 12-hydroxydehydrogenase, zinc binding alcohol dehydrogenase domain containing 3
- WB
Lab
Western blot - Human PTGR1 knockout HeLa cell lysate (AB258152)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : PTGR1 knockout HeLa cell lysate (20 ug)
ab181131 was shown to specifically react with PTGR1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265169 (knockout cell lysate ab258152) was used. Wild-type and PTGR1 knockout samples were subjected to SDS-PAGE. ab181131 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PTGR1 antibody [EPR13451-10] (<a href='/en-us/products/primary-antibodies/ptgr1-antibody-epr13451-10-ab181131'>ab181131</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PTGR1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PTGR1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ptgr1-knockout-hela-cell-line-ab265169'>ab265169</a>)
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 38 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PTGR1 knockout HeLa cell lysate (AB258152)
Allele-2 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human PTGR1 knockout HeLa cell lysate (AB258152)
Allele-1 : 2 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PTGR1 participates in metabolizing prostaglandins which play important roles in inflammation and homeostasis. It does not form part of a larger protein complex but works independently to control the levels of active prostaglandins within cells. This activity regulates physiological processes such as inflammation and vascular tone.
Pathways
Prostaglandin metabolism involves PTGR1's function. It integrates into the arachidonic acid pathway. Within this pathway PTGR1 interacts with other enzymes such as cyclooxygenases and lipoxygenases orchestrating the catabolism of prostaglandins and related lipid compounds. This coordination maintains the balance of inflammatory responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-PTGR1 antibody [EPR13451-10] (AB181131)](https://content.abcam.com/products/images/ptgr1-antibody-epr13451-10-ab181131--western-blot-img81676.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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