Human PTPN13 (FAP-1) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PTPN13 (FAP-1) knockout HeLa cell lysate (AB259070)
Homozygous : 4 bp deletion in exon14
Complementary Products
Primary Antibodies
AB207178
Anti-Fibroblast activation protein, alpha antibody [EPR20021]
BOND RX™ Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178)](https://content.abcam.com/products/images/fibroblast-activation-protein-alpha-antibody-epr20021-ab207178--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12529.png)
- 4
- 16
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PTPN13 plays a critical role in the regulation of apoptosis and cell-cell adhesion. It interacts with components of the cytoskeleton and other signaling proteins functioning as part of protein complexes involved in maintaining cellular structure and signaling. By dephosphorylating specific phosphotyrosine residues PTPN13 controls signaling pathways that determine cell survival and communication.
Pathways
PTPN13 is involved in pathways such as the Fas-mediated apoptosis signaling and insulin signaling pathways. It interacts with proteins like the Fas receptor and IRS1 within these pathways modulating signals that control apoptosis and metabolic responses. PTPN13's activity can affect downstream signaling events influencing cellular outcomes like survival or death in response to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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