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AB274957

Human PTPN2 knockout Jurkat cell lysate

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PTPN2 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%.
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Next Generation Sequencing - Human PTPN2 knockout Jurkat cell lysate (AB274957)
  • NGS

Supplier Data

Next Generation Sequencing - Human PTPN2 knockout Jurkat cell lysate (AB274957)

Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift : 100%

Key facts

Cell type

Jurkat

Species or organism

Human

Tissue

Blood

Knockout validation

Next Generation Sequencing

Mutation description

Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%

Disease

Non-Hodgkin Lymphoma

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Complementary Products

Primary Antibodies

AB129070

Anti-PTP1B + TCPTP antibody [EPR6712]

RabMAb|Recombinant|Trial Size

Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (AB129070)

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Proteins & Peptides

AB268903

Recombinant human TCPTP protein (Tagged)

Functional Studies - Recombinant human TCPTP protein (Tagged) (AB268903)

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Cell Lines & Lysates

AB274899

Human PTPN2 knockout Jurkat cell line

Advanced Validation

Next Generation Sequencing - Human PTPN2 knockout Jurkat cell line (AB274899)

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PTPN2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCPTP or T-cell protein tyrosine phosphatase is an enzyme that functions mechanically by removing phosphate groups from tyrosine residues on its substrate proteins. This process helps regulate various signaling pathways within cells. Also known as PTPN2 TCPTP is a protein with a mass of approximately 48 kDa. Its expression occurs in many tissues with notable levels in hematopoietic cells and within the endoplasmic reticulum.
Biological function summary

TCPTP plays a role in the control of cell growth differentiation and immune response. It functions independently not as part of a larger complex. This enzyme acts as a negative regulator of signaling pathways by dephosphorylating proteins that drive these cellular processes. Its activity ensures a balance in cellular signaling preventing overactivation which could lead to uncontrolled cell proliferation or inappropriate immune responses.

Pathways

TCPTP holds significant function in the JAK-STAT and insulin signaling pathways. Within the JAK-STAT pathway TCPTP dephosphorylates members such as JAK1 and JAK3 helping modulate cytokine responses and immune regulation. In the insulin signaling pathway it influences the dephosphorylation of the insulin receptor thereby impacting insulin sensitivity. Such interactions demonstrate its involvement in key cellular processes necessary for immune function and metabolic regulation.

TCPTP has associations with autoimmune diseases such as Type 1 Diabetes and Crohn’s Disease. Reduced activity or altered expression of TCPTP has been observed in these conditions suggesting its regulatory role in immune tolerance. Furthermore its connection with proteins like the cytokine IL-6 in autoimmune responses implicates TCPTP in the disease mechanisms where disrupted signaling can contribute to chronic inflammation and immune system dysfunction.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Suspension

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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