Human PTPRF (LAR) knockout HEK-293T cell lysate
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PTPRF KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4.
View Alternative Names
FLJ43335, FLJ45062, FLJ45567, LAR protein, LARFN5C, LARS, LCA homolog, Leukocyte antigen related, Leukocyte antigen related (LAR) PTP receptor, Leukocyte antigen related PTP receptor, Leukocyte antigen related tyrosine phosphatase, Leukocyte common antigen related, PTPRF protein, PTPRF_HUMAN, Protein Tyrosine Phosphatase Receptor Type F, Protein tyrosine phosphatase receptor type F polypeptide, Receptor linked protein tyrosine phosphatase LAR, Receptor type tyrosine protein phosphatase F precursor, Receptor-type tyrosine-protein phosphatase F
- Sanger seq
Unknown
Sanger Sequencing - Human PTPRF (LAR) knockout HEK-293T cell lysate (AB263319)
Allele-2 : 1 bp insertion in exon 4
- Sanger seq
Unknown
Sanger Sequencing - Human PTPRF (LAR) knockout HEK-293T cell lysate (AB263319)
Allele-1 : 5 bp deletion in exon 4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This receptor-like phosphatase supports important cell signaling functions. LAR is involved in neural development processes and is part of the synaptic adhesion complex. By interacting with proteins in the plasma membrane it helps modify and maintain neural connectivity necessary for proper nerve function. Its activity influences actin cytoskeleton dynamics and cell-cell adhesion.
Pathways
LAR plays a role in the axon guidance and insulin signaling pathways. In axon guidance it interacts with proteins such as netrin and nneural cell adhesion molecule (NCAM) to facilitate correct neural wiring. In the insulin signaling pathway LAR can dephosphorylate key proteins like insulin receptor affecting glucose metabolism and overall energy homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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