Human PTPRK knockout A549 cell lysate
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PTPRK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon5 and 4 bp deletion in exon5.
View Alternative Names
DKFZp686C2268, DKFZp779N1045, OTTHUMP00000017180, OTTHUMP00000017181, OTTHUMP00000017182, OTTHUMP00000017187, OTTHUMP00000040306, PTPK, PTPRK_HUMAN, PTPkappa, Protein tyrosine phosphatase kappa precursor, Protein tyrosine phosphatase receptor type K, Protein tyrosine phosphatase receptor type kappa, Protein-tyrosine phosphatase kappa, R-PTP-kappa, Receptor-type tyrosine-protein phosphatase kappa, dJ480J14.2.1
- Sanger seq
Unknown
Sanger Sequencing - Human PTPRK knockout A549 cell lysate (AB258155)
Allele-2 : 4 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human PTPRK knockout A549 cell lysate (AB258155)
Allele-1 : 10 bp deletion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PTPRK plays a role in regulating cell signaling by removing phosphate groups from phosphotyrosine residues on proteins. This activity influences cell growth differentiation and cellular communication. As a receptor-type protein tyrosine phosphatase it acts as a mediator in cell-cell adhesion and contact inhibition of cell growth. The protein is involved in the regulation of E-cadherin-mediated adhesion suggesting its importance in cell junctions.
Pathways
PTPRK is an important component in the Hedgehog and Wnt signaling pathways. These pathways are essential for embryonic development and maintenance of adult tissue homeostasis. PTPRK interacts with the calcium-dependent cell adhesion protein E-cadherin to mediate signal transduction within these pathways. The interplay with these pathways suggests its involvement in maintaining cellular architecture and function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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