PTPRK KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon5 and 4 bp deletion in exon5.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon5 and 4 bp deletion in exon5.
Alternative names
DKFZp686C2268, DKFZp779N1045, OTTHUMP00000017180, OTTHUMP00000017181, OTTHUMP00000017182, OTTHUMP00000017187, OTTHUMP00000040306, PTPK, PTPRK_HUMAN, PTPkappa, Protein tyrosine phosphatase kappa precursor, Protein tyrosine phosphatase receptor type K, Protein tyrosine phosphatase receptor type kappa, Protein-tyrosine phosphatase kappa, R-PTP-kappa, Receptor-type tyrosine-protein phosphatase kappa, dJ480J14.2.1
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PTPRK KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon5 and 4 bp deletion in exon5.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon5 and 4 bp deletion in exon5.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- PTPRK
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PTPRK also known as receptor-type tyrosine-protein phosphatase kappa is a protein involved in dephosphorylation processes. It has a molecular weight of approximately 145 kDa. The protein functions as a dimer and is encoded by the PTPRK gene. It is widely expressed in various tissues like the skin testes and colon. PTPRK interacts with other proteins at its extracellular region influencing cell adhesion and growth.
- Biological function summary
PTPRK plays a role in regulating cell signaling by removing phosphate groups from phosphotyrosine residues on proteins. This activity influences cell growth differentiation and cellular communication. As a receptor-type protein tyrosine phosphatase it acts as a mediator in cell-cell adhesion and contact inhibition of cell growth. The protein is involved in the regulation of E-cadherin-mediated adhesion suggesting its importance in cell junctions.
- Pathways
PTPRK is an important component in the Hedgehog and Wnt signaling pathways. These pathways are essential for embryonic development and maintenance of adult tissue homeostasis. PTPRK interacts with the calcium-dependent cell adhesion protein E-cadherin to mediate signal transduction within these pathways. The interplay with these pathways suggests its involvement in maintaining cellular architecture and function.
- Associated diseases and disorders
PTPRK has been associated with cancer and autoimmune diseases. Dysregulation of PTPRK expression or function can contribute to the development of colorectal cancer. Moreover altered PTPRK activity is implicated in psoriasis an autoimmune skin disorder where it is linked through an association with altered E-cadherin expression. These connections highlight the role of PTPRK in disease pathology and its potential as a therapeutic target.
Product promise
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2 product images
Sanger Sequencing - Human PTPRK knockout A549 cell lysate (ab258155)
Allele-2: 4 bp deletion in exon5
Sanger Sequencing - Human PTPRK knockout A549 cell lysate (ab258155)
Allele-1: 10 bp deletion in exon5
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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