Human PUM1 (Pumilio 1) knockout HeLa cell lysate
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PUM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 121 bp insertion in exon2 and 1 bp deletion in exon2.
View Alternative Names
HsPUM, KIAA0099, PUM L1, PUM1_HUMAN, PUMH, PUMH 1, PUML 1, PUML1, Pumilio homolog 1, Pumilio-1, pumilio homolog 1 (Drosophila)
- Sanger seq
Unknown
Sanger Sequencing - Human PUM1 (Pumilio 1) knockout HeLa cell lysate (AB257621)
Allele-2 : 121 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human PUM1 (Pumilio 1) knockout HeLa cell lysate (AB257621)
Allele-1 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PUM1 is an important regulator of gene expression. It functions by binding to the 3' untranslated regions of target mRNAs leading to repression of translation and in some cases RNA decay. PUM1 operates within larger ribonucleoprotein complexes collaborating with other regulatory proteins to fine-tune the stability and translation of specific transcripts. This regulation is vital for processes like neuronal development stem cell maintenance and germ cell development.
Pathways
The influence of PUM1 extends to key biological networks such as the cell cycle and long-term potentiation pathways. It interacts with proteins like DDX6 which synergize to mediate post-transcriptional regulation as part of the mRNA decay pathway. Through its role in these pathways PUM1 helps ensure precise control of gene expression necessary for cellular homeostasis and adaptive responses to environmental changes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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