Human PUS7 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
PUS7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.
View Alternative Names
FLJ20485, KIAA1897, MGC17720, OTTHUMP00000212074, OTTHUMP00000212075, OTTHUMP00000212076, PUS7_HUMAN, Pseudouridylate synthase 7 (putative), Pseudouridylate synthase 7 homolog, Pseudouridylate synthase 7 homolog (S. cerevisiae)
- Sanger seq
Unknown
Sanger Sequencing - Human PUS7 knockout HeLa cell lysate (AB259071)
Homozygous : 1 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PUS7 participates in the post-transcriptional modification of RNA which is critical for RNA maturation and function. This enzyme does not work in isolation; it interacts with other components of the RNA modification machinery. PUS7's activity enhances the structural stability of RNA molecules and affects the efficiency of protein synthesis by modifying RNA substrates fine-tuning their roles in processes like translation and splicing.
Pathways
PUS7 is involved in the RNA modification and processing pathways specifically those related to ribosome biogenesis and mRNA splicing. These pathways are central to the synthesis and maturation of various RNA species ensuring proper protein production. PUS7 interacts closely with proteins like fibrillarin in the nucleolus during rRNA processing and U2 auxiliary factor in mRNA splicing aligning with transcription dynamics and ribosome assembly.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB289857
Anti-PUS7 antibody [EPR25172-31]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody [EPR25172-31] (AB289857)](https://content.abcam.com/products/images/pus7-antibody-epr25172-31-ab289857--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164747.jpg)
- 5
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com