Human PVR (Poliovirus Receptor) knockout A549 cell lysate
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- WB
Lab
Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell lysate (AB261686)
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lane 2 : PVR knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lane 3 : U87-MG whole cell lysate 20 ug
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cell line ab261877 (knockout cell lysate ab261686). Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (<a href='/en-us/products/primary-antibodies/poliovirus-receptor-pvr-antibody-epr17302-ab205304'>ab205304</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
PVR knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-pvr-poliovirus-receptor-knockout-a549-cell-line-ab261877'>ab261877</a>)
Lane 3:
U87-MG whole cell lysate at 20 µg
Lane 4:
HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg
Predicted band size: 45 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human PVR (Poliovirus Receptor) knockout A549 cell lysate (AB261686)
Knockout achieved by CRISPR/Cas9; X = 11 bp deletion; Frameshift = 100%
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.
Pathways
PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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