Human PYHIN1 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PYHIN1 knockout A549 cell lysate (AB258613)
Allele-1 : 2 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human PYHIN1 knockout A549 cell lysate (AB258613)
Allele-3 : 8 bp insertion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human PYHIN1 knockout A549 cell lysate (AB258613)
Allele-2 : 2 bp insertion in exon5
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PYHIN1 contributes to immune system regulation and antiviral defense through nucleic acid sensing. It recognizes double-stranded DNA in the cytosol and interacts with other proteins to trigger the production of type I interferons and pro-inflammatory cytokines. PYHIN1 is not part of a large complex but interacts with other key proteins in the signaling pathway enhancing the immune response to viral infections and other pathogens.
Pathways
PYHIN1 is important in signaling pathways that activate the immune system such as the STING pathway and interferon regulatory factor pathways. PYHIN1 interacts with the cGAS-STING pathway detecting cytoplasmic DNA and facilitating signal transduction for innate immune activation. It is related to proteins like STING and IRF3 which are critical for transmitting signals leading to the production of interferons and other immune molecules.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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