Human QRICH1 (Glutamine-rich protein 1) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human QRICH1 (Glutamine-rich protein 1) knockout HeLa cell lysate (AB263323)
Allele-2 : Insertion of the selection cassette in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human QRICH1 (Glutamine-rich protein 1) knockout HeLa cell lysate (AB263323)
Allele-1 : 10 bp deletion in exon3
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
QRICH1 modulates cellular processes that involve gene expression. It is part of the transcriptional machinery complex interacting directly with other transcriptional regulators and co-activators. This interaction influences cellular activities such as proliferation differentiation and stress responses. QRICH1 regulates genes involved in cell cycle control and apoptosis affecting how cells respond to various internal and external signals. Its biological role makes it an important target for understanding gene regulatory networks.
Pathways
QRICH1 plays a role in pathways significant to cellular stress responses and apoptosis. It interacts with proteins such as p53 and E2F1 which are important in these pathways. QRICH1 helps mediate cellular responses to DNA damage working alongside p53 in the apoptotic signaling pathway. Additionally its interaction with transcription factors such as E2F1 highlights its involvement in cell cycle regulation and the balance between cell survival and apoptosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com