Human RAB10 knockout A549 cell lysate
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RAB10 KO cell lysate available now. KO validated by Immunocytochemistry, Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 100%.
View Alternative Names
GTP binding protein RAB10, RAB10 member RAS oncogene family, RAB10_HUMAN, Ras related GTP binding protein, Ras related GTP binding protein RAB10, Ras-related protein Rab-10
- WB
Lab
Western blot - Human RAB10 knockout A549 cell lysate (AB261677)
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lane 2 : RAB10 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab181367 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab181367 was shown to specifically react with RAB10 in wild-type A549 cells as signal was lost in RAB10 knockout cell line ab261868 (knockout cell lysate ab261677). Wild-type and RAB10 knockout samples were subjected to SDS-PAGE. ab181367 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-RAB10 antibody [EPR13242] (<a href='/en-us/products/primary-antibodies/rab10-antibody-epr13242-ab181367'>ab181367</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
RAB10 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
Western blot - Human RAB10 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-rab10-knockout-a549-cell-line-ab261868'>ab261868</a>)
Lane 3:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4:
MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Predicted band size: 22 kDa
Observed band size: 25 kDa
false
- NGS
Lab
Next Generation Sequencing - Human RAB10 knockout A549 cell lysate (AB261677)
1 bp insertion
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.
Pathways
RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB237703
20ul selling size|KO Validated|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [MJF-R23] (AB237703)](https://content.abcam.com/products/images/rab10-antibody-mjf-r23-ab237703--immunocytochemistry-immunofluorescence-img422296.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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