Human RAB14 knockout HEK-293T cell lysate
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RAB14 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
View Alternative Names
F protein binding protein 1, FBP, GTPase Rab14, RAB14 member RAS oncogene family, RAB14_HUMAN, RP11 165P4.4, Ras-related protein Rab-14, Small GTP binding protein RAB14, bA165P4.3
- Sanger seq
Unknown
Sanger Sequencing - Human RAB14 knockout HEK-293T cell lysate (AB258160)
Allele-1 : Insertion of the selection cassette in exon 4
- Sanger seq
Unknown
Sanger Sequencing - Human RAB14 knockout HEK-293T cell lysate (AB258160)
Allele-2 : 2 bp insertion in exon 4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RAB14 orchestrates intracellular transport processes important for cell homeostasis. It does not act alone but often forms complexes with other proteins like protein phosphatase 1 and interactions involving KIF3A and DVL. These interactions enable RAB14 to influence cargo sorting and organelle morphology which is essential for correct protein trafficking and recycling in the cells.
Pathways
Scientists have placed RAB14 in relation to the regulation of membrane trafficking and cell signaling. RAB14 influences the pathways of endocytosis and trans-Golgi network trafficking. It has associations with proteins such as RAB11 and RAB5 in these pathways. These proteins work in coordination to ensure effective sorting and distribution of intracellular materials affecting various cellular processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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