RAB14 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
Alternative names
F protein binding protein 1, FBP, GTPase Rab14, RAB14 member RAS oncogene family, RAB14_HUMAN, RP11 165P4.4, Ras-related protein Rab-14, Small GTP binding protein RAB14, bA165P4.3
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RAB14 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
- Concentration
Properties
- Gene name
- RAB14
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB14 also known as Ras-related protein Rab-14 is part of the Rab family of small GTPases. It has a molecular mass of approximately 23 kDa and functions to regulate membrane trafficking between the Golgi apparatus and endosomes. RAB14 mediates vesicle movement and membrane fusion playing an important role in endocytic and secretory pathways. It is expressed in various tissues and cells including the brain heart and lungs suggesting its involvement in diverse physiological processes.
- Biological function summary
RAB14 orchestrates intracellular transport processes important for cell homeostasis. It does not act alone but often forms complexes with other proteins like protein phosphatase 1 and interactions involving KIF3A and DVL. These interactions enable RAB14 to influence cargo sorting and organelle morphology which is essential for correct protein trafficking and recycling in the cells.
- Pathways
Scientists have placed RAB14 in relation to the regulation of membrane trafficking and cell signaling. RAB14 influences the pathways of endocytosis and trans-Golgi network trafficking. It has associations with proteins such as RAB11 and RAB5 in these pathways. These proteins work in coordination to ensure effective sorting and distribution of intracellular materials affecting various cellular processes.
- Associated diseases and disorders
Mutations or dysregulation of RAB14 associate with cancer progression and metabolic disorders. Abnormal RAB14 activity influences tumorigenesis by affecting cell migration and invasion in part through interactions with proteins such as Rac1. Additionally RAB14 plays a role in insulin signaling pathways linking it to metabolic disorders related to insulin resistance and glucose homeostasis.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human RAB14 knockout HEK-293T cell lysate (ab258160)
Allele-1: Insertion of the selection cassette in exon 4
Sanger Sequencing - Human RAB14 knockout HEK-293T cell lysate (ab258160)
Allele-2: 2 bp insertion in exon 4
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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