Human RAB20 knockout HeLa cell lysate
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RAB20 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon1 and 28 bp deletion in exon1 and 34 bp deletion in exon1.
View Alternative Names
FLJ20429, RAB20 member RAS oncogene family, RAB20_HUMAN, Ras-related protein Rab-20
- Sanger seq
Unknown
Sanger Sequencing - Human RAB20 knockout HeLa cell lysate (AB258616)
Allele-2 : 22 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RAB20 knockout HeLa cell lysate (AB258616)
Allele-3 : 34 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RAB20 knockout HeLa cell lysate (AB258616)
Allele-1 : 28 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rab20 regulates intracellular membrane transport processes. It is involved in endocytic and exocytic transport pathways by controlling vesicle formation movement and fusion. Rab20 participates in the recycling of endosomes contributing to the homeostasis of cellular components. Rab20 is also involved in a complex with other Rab proteins modulating their specific functions in vesicle trafficking.
Pathways
Rab20 plays a role in the endocytic pathway specifically in the recycling endosome pathway where it acts alongside proteins such as Rab11. Rab20 supports the retrieval and recycling of receptors from endosomes back to the plasma membrane. Related to this function Rab20 associates with the exocytic pathway ensuring proper transport of cargo proteins from the Golgi apparatus to the cell surface.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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