Human RAB2A (Rab2) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human RAB2A (Rab2) knockout HEK-293T cell lysate (AB258620)
Homozygous : 1 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ras-related protein Rab-2 mediates the trafficking of proteins and lipids within the secretory pathway. Rab2 associates with the cytosolic face of membranes aiding in the formation and movement of vesicles. It forms part of a complex that regulates tethering and docking facilitating vesicle fusion. Rab2 interaction with its effectors ensures accurate delivery of cargo to the correct cellular compartments. This functionality supports the maintenance of cellular organization and communication.
Pathways
Ras-related protein Rab-2 functions in the ER-Golgi transport pathway and the autophagy pathway. In the ER-Golgi transport pathway it interacts with proteins such as Golgi SNAREs to enhance vesicle sorting and fusion. Rab2 also has connections with the autophagy-related proteins where it supports the formation of autophagosomes therefore contributing to cellular homeostasis and turnover. Its activity impacts the coordination between membrane trafficking and recycling processes within cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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