Human RAB6A (Rab-6) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human RAB6A (Rab-6) knockout HEK-293T cell lysate (AB258621)
Homozygous : 2 bp deletion in exon 1
Complementary Products
Primary Antibodies
AB271094
Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size
![Multiplex immunohistochemistry - Anti-Rab 6A + Rab 6D + Ral-A antibody [EPR24472-24] (AB271094)](https://content.abcam.com/products/images/rab-6a-rab-6d-ral-a-antibody-epr24472-24-ab271094--multiplex-immunohistochemistry-img439220.jpg)
- 5
- 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rab-6 aids in the coordination of retrograde vesicle transport from the Golgi to the endoplasmic reticulum. Rab-6 functions as part of a complex with other proteins including dynactin and cytoplasmic dynein to facilitate these movements. Its role includes ensuring proper delivery and sorting of proteins within the cell which is essential for maintaining cellular organization and function.
Pathways
This protein is involved in the endocytosis and secretory pathways. In the endocytosis pathway Rab-6 interacts with proteins like Rab GDI and Rab escort protein to regulate vesicle transport. In the secretory pathway the protein ensures that cargo proteins transit correctly through the Golgi interacting with other Rab family members such as Rab-1 which also play parts in vesicle movement and secretion processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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