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AB263831

Human RAB7A (RAB7) knockout HeLa cell lysate

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RAB7A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon2.

View Alternative Names

CMT2B, PRO2706, PSN, RAB7, member RAS oncogene family, RAB7A, member RAS oncogene family, RAB7A_HUMAN, Ras associated protein RAB7, Ras related protein Rab7, Ras-related protein Rab-7a

3 Images
Western blot - Human RAB7A (RAB7) knockout HeLa cell lysate (AB263831)
  • WB

Lab

Western blot - Human RAB7A (RAB7) knockout HeLa cell lysate (AB263831)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : RAB7A knockout HeLa cell lysate 20 μg
Lanes 1 - 2 : Merged signal (red and green). Green - ab137029 observed at 23 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab137029 was shown to react with RAB7 in wild-type HeLa cells in Western blot with loss of signal observed in RAB7A knockout cell line ab255423 (RAB7A knockout cell lysate ab263831). Wild-type HeLa and RAB7A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab137029 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (<a href='/en-us/products/primary-antibodies/rab7-antibody-epr7589-late-endosome-marker-ab137029'>ab137029</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RAB7A knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RAB7A (RAB7) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rab7a-rab7-knockout-hela-cell-line-ab255423'>ab255423</a>)

Observed band size: 23 kDa

false

Western blot - Human RAB7A (RAB7) knockout HeLa cell lysate (AB263831)
  • WB

Lab

Western blot - Human RAB7A (RAB7) knockout HeLa cell lysate (AB263831)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : RAB7A knockout HeLa cell lysate 20 μg
Lanes 1 - 2 : Merged signal (red and green). Green - ab126712 observed at 23 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab126712 was shown to react with RAB7 in wild-type HeLa cells in Western blot with loss of signal observed in RAB7A knockout cell line ab255423 (RAB7A knockout cell lysate ab263831). Wild-type HeLa and RAB7A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab126712 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (<a href='/en-us/products/primary-antibodies/rab7-antibody-epr7588b-late-endosome-marker-ab126712'>ab126712</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RAB7A knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RAB7A (RAB7) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rab7a-rab7-knockout-hela-cell-line-ab255423'>ab255423</a>)

Observed band size: 23 kDa

false

Sanger Sequencing - Human RAB7A (RAB7) knockout HeLa cell lysate (AB263831)
  • Sanger seq

Unknown

Sanger Sequencing - Human RAB7A (RAB7) knockout HeLa cell lysate (AB263831)

Homozygous : 25 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon2.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
RAB7A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB7 also known as RAB7A is a small GTPase belonging to the RAS oncogene family. It has a molecular mass of approximately 23 kDa. This protein plays an important role in the regulation of late endocytic trafficking. It is prominently expressed in the cytoplasmic compartments of various cell types and serves as an important late endosome marker. RAB7 controls the maturation of early endosomes into late endosomes and their subsequent fusion with lysosomes therefore influencing degradation pathways.
Biological function summary

RAB7 affects various processes including autophagy cell signaling and pathogen infection response. It often functions as part of the endosomal machinery and forms complexes with other proteins like RAB3GAP1 and RILP. These complexes help mediate vesicle transport and membrane trafficking processes which are essential for the maintenance of cellular homeostasis. RAB7 is significant in ensuring proper lysosomal positioning and function which is critical for cellular metabolism and degradation.

Pathways

RAB7 integrates into the endocytic and autophagic pathways by facilitating the transport between endosomes and lysosomes. It connects with the PI3K/AKT pathway impacting cell proliferation and survival. RAB7 also interacts with the retromer complex influencing the retrieval of receptors from endosomes. Proteins like FYCO1 and Prostaglandin E2 synthase can also cooperate with RAB7 in these pathways further establishing its role in cellular dynamics and signaling.

RAB7 has associations with Charcot-Marie-Tooth disease type 2B and cancer progression. Mutations in the RAB7 gene have been linked to neuropathic conditions leading to impaired motor and sensory function. In the context of cancer dysregulation of RAB7 expression can contribute to increased tumor growth and metastasis. RAB7 interacts with proteins such as MAPK and ARL8B which are involved in these diseases highlighting its importance in pathological conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB137029

Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB137029)

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Proteins & Peptides

AB103507

Recombinant Human RAB7 protein

SDS-PAGE - Recombinant Human RAB7 protein (AB103507)

  • 0
  • 0
Cell Lines & Lysates

AB255423

Human RAB7A (RAB7) knockout HeLa cell line

Advanced Validation

Western blot - Human RAB7A (RAB7) knockout HeLa cell line (AB255423)

  • 0
  • 0

Product promise

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