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RAB9A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.

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Images

Western blot - Human RAB9A (Rab9) knockout HeLa cell lysate (AB257625), expandable thumbnail
  • Western blot - Human RAB9A (Rab9) knockout HeLa cell lysate (AB257625), expandable thumbnail
  • Sanger Sequencing - Human RAB9A (Rab9) knockout HeLa cell lysate (AB257625), expandable thumbnail
  • Sanger Sequencing - Human RAB9A (Rab9) knockout HeLa cell lysate (AB257625), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.

Alternative names

2410064E05Rik, AI195561, DmRab9, RAB9 member RAS oncogene family, RAB9A member RAS oncogene family, RAB9A_HUMAN, RAS ASSOCIATED PROTEIN RAB9, Rab 9, Ras-related protein Rab-9A, Sid6061p, Sid99

What's included?

1 Kit
Components
Human RAB9A knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

RAB9A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.

Key facts

Cell type
HeLa
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
RAB9A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab9 also known as Ras-related protein Rab-9A is a small GTPase and part of the Rab family with a mass of around 23 kDa. This protein mainly resides in the trans-Golgi network and late endosomes. Rab9 plays an important role in the transport of mannose 6-phosphate receptors between late endosomes and the Golgi apparatus facilitating cargo sorting and vesicular trafficking in cells.

Biological function summary

Rab9 coordinates several intracellular trafficking processes aiding in the regulation of membrane identity. It associates with other effectors such as tail-interacting protein (TIP47) to form complexes on the endosomal surface. These complexes help in vesicular transport ensuring efficient recycling of receptors. Rab9 impacts endosome maturation and transport making it an essential endosome marker.

Pathways

Rab9 influences and is involved in the regulation of the endocytic and secretory pathways. It interacts with proteins like Rab7 another GTPase to manage late endosome and lysosome transport. These interactions underline Rab9's functionalities in intracellular trafficking and recycling processes relying on cooperative interactions with other Rabs and their effectors.

Associated diseases and disorders

Rab9 plays a role in conditions like Niemann-Pick disease type C and some neurological disorders. Disruption in Rab9 function may affect lipid trafficking and lead to accumulation of cholesterol in late endosomes. This links Rab9 indirectly to proteins such as NPC1 which is known to be implicated in cholesterol homeostasis. Understanding Rab9's involvement can help inform therapeutic strategies targeting these pathways in related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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