Human RABAC1 (PRA1) knockout HeLa cell lysate
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RABAC1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
View Alternative Names
PRA1 domain family 1, PRA1 family protein 1, PRAF1_HUMAN, Prenylated Rab acceptor protein 1, RABAC1, Rab acceptor 1 (prenylated), YIP3, prenylated Rab acceptor 1
- Sanger seq
Unknown
Sanger Sequencing - Human RABAC1 (PRA1) knockout HeLa cell lysate (AB258162)
Homozygous : 1 bp deletion in exon1
- WB
Lab
Western blot - Human RABAC1 (PRA1) knockout HeLa cell lysate (AB258162)
Lanes 1-3 : Merged signal (red and green). Green - ab76413 observed at 21 kDa. Red - loading control ab7291 observed at 50 kDa.
ab76413 Anti-PRA1 antibody [EPR1747Y] was shown to specifically react with PRA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265702 (knockout cell lysate ab258162) was used. Wild-type and PRA1 knockout samples were subjected to SDS-PAGE. ab76413 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PRA1 antibody [EPR1747Y] (<a href='/en-us/products/primary-antibodies/pra1-antibody-epr1747y-ab76413'>ab76413</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RABAC1 (PRA1) knockout HeLa cell lysate (ab258162) at 20 µg
Lane 3:
HEK293 cell lysate at 20 µg
Secondary
Lanes 1 - 3:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Lanes 1 - 3:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa,50 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PRA1 interacts with Rab GTPases which suggest its role in facilitating the docking or fusion of transport vesicles with target membranes. Although it does not form a complex on its own PRA1 acts as an effector anchoring prenylated Rab proteins. This interaction is essential for maintaining the fidelity and specificity of vesicle transport events necessary for proper cellular functioning.
Pathways
PRA1 participates in the regulation of the Rab GTPase cycle and is important in the endocytic and secretory pathways. It modulates the activity of proteins such as Rab1 and Rab5 that coordinate the movement of vesicles from the endoplasmic reticulum to the Golgi and from the Golgi to the plasma membrane. These pathways are fundamental for cellular logistics ensuring that proteins reach the intracellular or extracellular locations where they are needed.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-PRA1 antibody [EPR1747Y] (AB76413)](https://content.abcam.com/products/images/pra1-antibody-epr1747y-ab76413--western-blot-img43316.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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