Human RAD1 knockout HeLa cell lysate
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RAD1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2 and 62 bp deletion in exon2.
View Alternative Names
Cell cycle checkpoint protein Hrad1, Cell cycle checkpoint protein RAD1, Cell cycle checkpoint protein Rad 1 A / B, Checkpoint control protein HRAD1, Checkpoint control protein RAD1, DNA repair exonuclease, DNA repair exonuclease REC1, DNA repair exonuclease rad1, DNA repair exonuclease rad1 homolog, DNA repair protein RAD1, EC 3.1.11.2, Exonuclease homolog RAD1, GTP-binding protein RAD, MGC77779, RAD1 homolog, RAD1 homolog (S. pombe), RAD1, S. pombe, homolog of, RAD1_HUMAN, REC 1, Rad1 like DNA damage checkpoint, Rad1-like DNA damage checkpoint protein, Ras associated with diabetes, hRAD 1
- Sanger seq
Unknown
Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)
Allele-3 : 62 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)
Allele-1 : 2 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rad1 plays an essential role in the DNA damage response by being part of the 9-1-1 complex which includes Rad9 and Hus1 proteins. This complex is an important player in activating the ATR signaling pathway which helps cells respond to DNA replication stress and damage. Rad1 helps ensure proper checkpoint activation preventing cells with damaged DNA from progressing through the cell cycle which further safeguards genomic integrity.
Pathways
The protein Rad1 functions critically within the ATR-Chk1 signaling pathway a significant route for controlling cell cycle checkpoints. In this context Rad1 associates with proteins such as ATR and Chk1 facilitating the signaling necessary for cell cycle arrest under conditions of DNA stress. Rad1 also interacts with the base excision repair pathway where it works with other repair proteins to rectify oxidative DNA damage and maintain cellular viability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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