Human RALBP1 knockout HeLa cell lysate
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RALBP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.
View Alternative Names
76-kDa Ral-interacting protein, DNP-SG ATPase, Dinitrophenyl S-glutathione ATPase, RBP1_HUMAN, RLIP1, RLIP76, Ral-interacting protein 1, Ral-interacting protein 1, 76-KD, RalA-binding protein 1, Rip-1
- WB
Lab
Western blot - Human RALBP1 knockout HeLa cell lysate (AB258167)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : RALBP1 knockout HeLa cell lysate (20 μg)
Lane 3 : Daudi cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab133549 observed at 95 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133549 Anti-RALBP1 antibody [EPR6472] was shown to specifically react with PDE10A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265404 (knockout cell lysate ab258167) was used. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab133549 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-RALBP1 antibody [EPR6472] (<a href='/en-us/products/primary-antibodies/ralbp1-antibody-epr6472-ab133549'>ab133549</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RALBP1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RALBP1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ralbp1-knockout-hela-cell-line-ab265404'>ab265404</a>)
Lane 3:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa
false
- WB
Unknown
Western blot - Human RALBP1 knockout HeLa cell lysate (AB258167)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : RALBP1 knockout HeLa cell lysate (20 μg)
Lane 3 : HAP1 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab33446 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.
ab33446 Anti-RALBP1 antibody was shown to specifically react with PDE10A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265404 (knockout cell lysate ab258167) was used. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab33446 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Anti-RALBP1 antibody (<a href='/en-us/products/unavailable/ralbp1-antibody-ab33446'>ab33446</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RALBP1 knockout HeLa cell lysate at 20 µg
Lane 3:
HAP1 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
false
- Sanger seq
Unknown
Sanger Sequencing - Human RALBP1 knockout HeLa cell lysate (AB258167)
Homozygous : 1 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RALBP1 serves as an effector protein involved in several cellular activities including endocytosis and transport of glutathione conjugates. It functions as part of a complex with partner proteins facilitating the movement of substrates across cellular membranes. This role is essential for maintaining cellular homeostasis as it helps to eliminate toxic compounds and regulate intracellular signals.
Pathways
RALBP1 participates in the Rho family small GTPase signaling and the Ras signaling pathways. Within these pathways the protein coordinates with RalA and other GTPases to control the trafficking of vesicles and stress response mechanisms. Through these interactions RALBP1 plays an important role in cytoskeletal reorganization and cellular response to extracellular cues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB248548
Anti-RALBP1 antibody [EPR6472] - BSA and Azide free
RabMAb|Recombinant|KO Validated
![Western blot - Anti-RALBP1 antibody [EPR6472] - BSA and Azide free (AB248548)](https://content.abcam.com/products/images/ralbp1-antibody-epr6472-bsa-and-azide-free-ab248548--western-blot-img5544.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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