Human RARA (Retinoic Acid Receptor alpha) knockout HeLa cell lysate
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RARA KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7.
View Alternative Names
NR1B1, Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein, Nuclear receptor subfamily 1 group B member 1, Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form, RAR, RAR-alpha, RARA_HUMAN, RARalpha1, Retinoic acid nuclear receptor alpha variant 1, Retinoic acid nuclear receptor alpha variant 2, Retinoic acid receptor alpha, Retinoic acid receptor alpha polypeptide
- WB
Lab
Western blot - Human RARA (Retinoic Acid Receptor alpha) knockout HeLa cell lysate (AB257629)
Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : RARA knockout HeLa cell lysate 20 ug
Lane 3 : MCF7 cell lysate 20 ug
Lane 4 : HepG2 cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab275745 observed at 40 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.#ab275745 was shown to react with Retinoic Acid Receptor alpha in wild-type HeLa cells in western blot. The bands observed in RARA CRISPR-Cas9 edited cell line ab265176 (RARA CRISPR-Cas9 edited cell lysate ab257629) below 40 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and RARA CRISPR-Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab275745 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (<a href='/en-us/products/primary-antibodies/retinoic-acid-receptor-alpha-antibody-epr23871-271-ab275745'>ab275745</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RARA CRISPR-Cas9 edited HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RARA (Retinoic Acid Receptor alpha) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rara-retinoic-acid-receptor-alpha-knockout-hela-cell-line-ab265176'>ab265176</a>)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Predicted band size: 51 kDa
Observed band size: 50-55 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RARA (Retinoic Acid Receptor alpha) knockout HeLa cell lysate (AB257629)
Homozygous : 1 bp insertion in exon7
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RARA plays an essential role in mediating the effects of retinoic acid in the body. It is part of a larger receptor complex that interacts with co-regulators to modulate gene expression. This process is significant for embryonic development and the maintenance of normal physiological functions. Through its action RARA contributes to the proper development of organs and is critical for maintaining immune homeostasis and enabling the cellular response to environmental changes.
Pathways
RARA's activity impacts important signaling routes such as the retinoic acid signaling pathway and the Wnt signaling pathway. It collaborates with proteins like retinoid X receptors (RXRs) and other nuclear receptors to influence gene expression processes. These pathways maintain cellular differentiation and tissue homeostasis demonstrating RARA's integrative role in cellular signaling and communication.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB125001
Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]
RabMAb|Recombinant|KO Validated
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- 5
- 5
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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