Human RARRES3 (TIG3) knockout A549 cell lysate
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PLAAT4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3 and 4 bp deletion in exon3.
View Alternative Names
HRASLS4, MGC8906, RAR-responsive protein TIG3, RARRES3, Retinic acid inducible gene 1, Retinoic acid receptor responder (tazarotene induced) 3, Retinoic acid receptor responder protein 3, Retinoid-inducible gene 1 protein, Rig-1, TIG3_HUMAN, Tazarotene-induced gene 3 protein
- Sanger seq
Unknown
Sanger Sequencing - Human RARRES3 (TIG3) knockout A549 cell lysate (AB258625)
Allele-3 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human RARRES3 (TIG3) knockout A549 cell lysate (AB258625)
Allele-1 : 4 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human RARRES3 (TIG3) knockout A549 cell lysate (AB258625)
Allele-2 : 2 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TIG3 regulates cellular differentiation and promotes apoptosis. It does not form part of a larger complex but interacts with several key proteins in cell cycle control. TIG3 has an important role in maintaining the balance between cell proliferation and apoptosis particularly in keratinocytes. This regulatory action is important in skin health and response to external signals which might include pharmaceutical agents like retinoids.
Pathways
TIG3 acts primarily in the retinoic acid signaling pathway. This pathway involves its interaction with retinoic acid receptors such as RAR and RXR which regulate gene expression. These interactions influence the transcriptional activity of genes controlling cell differentiation and growth arrest. Additionally TIG3 has a link with the apoptosis pathway where it mediates the expression of proteins like p53 a well-known tumor suppressor that can induce cell cycle arrest and apoptosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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