JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258628

Human RBBP7 (RbAp46) knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

RBBP7 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

View Alternative Names

G1/S transition control protein binding protein RbAp46, Histone acetyltransferase type B subunit 2, Histone-binding protein RBBP7, MGC138867, MGC138868, Nucleosome-remodeling factor subunit RBAP46, RBAP46, RBBP7_HUMAN, Retinoblastoma-binding protein 7, Retinoblastoma-binding protein RbAp46, Retinoblastoma-binding protein p46

4 Images
Western blot - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)
  • WB

Lab

Western blot - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)

Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : RBBP7 knockout HeLa cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab259957 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab259957 was shown to react with RBBP7 in wild-type HeLa cells in Western blot with loss of signal observed in RBBP7 knockout cell line ab264677 (RBBP7 knockout cell lysate ab258628). Wild-type HeLa and RBBP7 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab259957 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (<a href='/en-us/products/primary-antibodies/rbbp7-antibody-epr23796-74-chip-grade-ab259957'>ab259957</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RBBP7 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RBBP7 (RbAp46) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rbbp7-rbap46-knockout-hela-cell-line-ab264677'>ab264677</a>)

Predicted band size: 48 kDa

Observed band size: 50 kDa

false

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)
  • Sanger seq

Unknown

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)

Allele-2 : 1 bp deletion in exon 2

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)
  • Sanger seq

Unknown

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)

Allele-1 : 4 bp deletion in exon 2

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)
  • Sanger seq

Unknown

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HeLa cell lysate (AB258628)

Allele-3 : Insertion of the selection cassette in exon 2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

Disease

Adenocarcinoma

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258628-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human RBBP7 knockout HeLa cell lysate", "number":"AB258628-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258628-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
RBBP7
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB259957

Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Western blot - Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade (AB259957)

  • 0
  • 0
Proteins & Peptides

AB114396

Recombinant Human RBBP7 protein

SDS-PAGE - Recombinant Human RBBP7 protein (AB114396)

  • 0
  • 0
Cell Lines & Lysates

AB266877

Human RBBP7 (RbAp46) knockout HCT116 cell line

Sanger Sequencing - Human RBBP7 (RbAp46) knockout HCT116 cell line (AB266877)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com