Human RBL1 (p107) knockout HeLa cell lysate
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RBL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon9.
View Alternative Names
107 kDa retinoblastoma-associated protein, CP107, Cellular protein 107, Cellular protein p107, MGC40006, PRB1, RBL1_HUMAN, Retinoblastoma-like protein 1, p107, retinoblastoma-like 1 (p107)
- WB
Lab
Western blot - Human RBL1 (p107) knockout HeLa cell lysate (AB258630)
Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : RBL1 knockout HeLa cell lysate 20 ug
Lane 3 : HCT 116 cell lysate 20 ug
Lane 4 : MCF7 cell lysate 20 ug
False colour image of Western blot : Anti-p107 antibody staining at 1/400 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245514 was shown to bind specifically to p107. A band was observed at 121 kDa in wild-type HeLa cell lysates with no signal observed at this size in RBL1 knockout cell line ab264706 (knockout cell lysate ab258630). To generate this image, wild-type and RBL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-p107 antibody (<a href='/en-us/products/primary-antibodies/p107-antibody-ab245514'>ab245514</a>) at 1/400 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RBL1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RBL1 (p107) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rbl1-p107-knockout-hela-cell-line-ab264706'>ab264706</a>)
Lane 3:
HCT 116 cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
Predicted band size: 121 kDa
Observed band size: 121 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RBL1 (p107) knockout HeLa cell lysate (AB258630)
Homozygous : 1 bp insertion in exon9
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Retinoblastoma-like protein 1 functions in the regulation of the cell cycle acting as an important checkpoint controller between the G1 and S phases. This protein forms a complex with E2F transcription factors which are important in controlling genes involved in cell cycle progression. The p107-E2F interaction represses transcription and inhibits the cell from proceeding to the S phase thereby ensuring proper cellular proliferation control. Moreover p107 can bind to cyclin-dependent kinases adding another layer of regulation.
Pathways
P107 plays essential roles in the RB-E2F pathway and the DNA damage response pathway. Within these pathways p107 interacts with other key proteins like p130 and pRB which together form the pocket protein family. The RB-E2F pathway controls cell cycle entry and exit while the DNA damage response pathway ensures genomic integrity. p107 acts as a mediator to pause the cell cycle during DNA damage repair collaborating with these related proteins to maintain cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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