Human RBM3 knockout HEK-293T cell lysate
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RBM3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2.
View Alternative Names
2600016C11Rik, IS1 RNPL, MGC105811, MGC118410, OTTHUMP00000025800, OTTHUMP00000025802, OTTMUSP00000019634, OTTMUSP00000019635, OTTMUSP00000019636, Putative RNA-binding protein 3, RBM3_HUMAN, RNA binding motif (RNP1, RRM) protein 3, RNA-binding motif protein 3, RNA-binding protein 3, RNPL, RP23-27I6.7
- WB
Lab
Western blot - Human RBM3 knockout HEK-293T cell lysate (AB258170)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : RBM3 Knockout HEK-293T cell lysate 20 μg
Lane 3 : Hela cell lysate 20 μg
Lane 4 : LnCap cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab134946 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab134946 was shown to react with RBM3 in wild-type HEK-293T cells in Western blot with loss of signal observed in RBM3 knockout cell line ab266663 (RBM3 knockout cell lysate ab258170). Wild-type HEK-293T and RBM3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134946 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-RBM3 antibody [EPR6061(2)] (<a href='/en-us/products/primary-antibodies/rbm3-antibody-epr60612-ab134946'>ab134946</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
RBM3 Knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human RBM3 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-rbm3-knockout-hek-293t-cell-line-ab266663'>ab266663</a>)
Lane 3:
Hela cell lysate at 20 µg
Lane 4:
LnCap cell lysate at 20 µg
Predicted band size: 17 kDa
Observed band size: 18 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RBM3 knockout HEK-293T cell lysate (AB258170)
Homozygous : 2 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RBM3 functions by modulating RNA stability and translation impacting cellular stress responses. It forms part of ribonucleoprotein complexes influencing the processing of several RNA species. The protein enhances mRNA stability under stress conditions and promotes cell survival by impacting protein synthesis. RBM3 also participates in synaptic plasticity which is essential for learning and memory showcasing its extensive influence on cellular functioning.
Pathways
RBM3 significantly contributes to neuroprotection and anti-apoptosis pathways. It is closely linked to pathways dealing with stress granules and mRNA stabilization. A known association exists between RBM3 and proteins such as CIRP (cold-inducible RNA-binding protein) which share similar pathways concerning mRNA regulation under cold conditions. This connection highlights RBM3's role in cell survival and stress management.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB134946
Anti-RBM3 antibody [EPR6061(2)]
BOND RX™ Validated|KO Validated|RabMAb|Recombinant
![Flow Cytometry (Intracellular) - Anti-RBM3 antibody [EPR6061(2)] (AB134946)](https://content.abcam.com/products/images/rbm3-antibody-epr60612-ab134946--flow-cytometry-intracellular-img38662.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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