Human RBPMS knockout HeLa cell lysate
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RBPMS KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 44 bp deletion in exon2.
View Alternative Names
FLJ32971, Heart and RRM expressed sequence, Hermes, RBPMS_HUMAN, RNA binding protein gene with multiple splicing, RNA-binding protein with multiple splicing
- WB
Lab
Western blot - Human RBPMS knockout HeLa cell lysate (AB258631)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : RBPMS Knockout HeLa cell lysate 20 μg
Lane 3 : RT-4 cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab194213 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab194213 was shown to react with RBPMS in wild-type HeLa cells in western blot. Here no band was observed in the RBPMS knockout cell line ab264697 (RBPMS knockout cell lysate ab258631), but other antibodies have shown bands in this lysate below 30 kDa that may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab194213 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 4:
Western blot - Anti-RBPMS antibody (<a href='/en-us/products/primary-antibodies/rbpms-antibody-ab194213'>ab194213</a>) at 1/1000 dilution
Lanes 1 - 4:
Western blot - Anti-RBPMS antibody (<a href='/en-us/products/primary-antibodies/rbpms-antibody-ab152101'>ab152101</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RBPMS Knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RBPMS knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rbpms-knockout-hela-cell-line-ab264697'>ab264697</a>)
Lane 3:
RT-4 cell lysate at 20 µg
Lane 4:
U-251 MG cell lysate at 20 µg
Predicted band size: 22 kDa
Observed band size: 30 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RBPMS knockout HeLa cell lysate (AB258631)
Allele-1 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human RBPMS knockout HeLa cell lysate (AB258631)
Allele-2 : 44 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.
Pathways
RBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB309094
Anti-RBPMS antibody [EPR27130-21]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation
![Flow Cytometry (Intracellular) - Anti-RBPMS antibody [EPR27130-21] (AB309094)](https://content.abcam.com/products/images/rbpms-antibody-epr27130-21-ab309094--flow-cytometry-intracellular-img409585.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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