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AB258172

Human RCN1 (RCN) knockout HEK-293T cell lysate

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RCN1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

View Alternative Names

Epididymis secretory protein Li 84, FLJ37041, HEL S 84, PIG20, Proliferation inducing gene 20, RCAL, RCN, RCN1_HUMAN, Reticulocalbin 1 EF hand calcium binding domain, Reticulocalbin-1

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Western blot - Human RCN1 (RCN) knockout HEK-293T cell lysate (AB258172)
  • WB

Lab

Western blot - Human RCN1 (RCN) knockout HEK-293T cell lysate (AB258172)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : RCN1 knockout HEK-293T cell lysate (20µg)

Lane 3 : HeLa cell lysate (20µg)

Lane 4 : Daudi cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab210404 observed at 45 kDa. Red - loading control ab7291 observed at 50 kDa.

ab210404 Anti-RCN1/RCN antibody [EPR17163-117] was shown to specifically react with RCN1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266098 (knockout cell lysate ab258172) was used. Wild-type and RCN1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab210404 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RCN1/RCN antibody [EPR17163-117] (<a href='/en-us/products/primary-antibodies/rcn1-rcn-antibody-epr17163-117-ab210404'>ab210404</a>) at 1/2000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

RCN1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human RCN1 (RCN) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-rcn1-rcn-knockout-hek-293t-cell-line-ab266098'>ab266098</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 39 kDa

Observed band size: 45 kDa

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Sanger Sequencing - Human RCN1 (RCN) knockout HEK-293T cell lysate (AB258172)
  • Sanger seq

Unknown

Sanger Sequencing - Human RCN1 (RCN) knockout HEK-293T cell lysate (AB258172)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
RCN1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RCN1 also known as reticulocalbin-1 or RCN is a calcium-binding protein located in the lumen of the endoplasmic reticulum (ER). It belongs to the CREC family of proteins which are characterized by their EF-hand motifs that mediate calcium binding. RCN1 has a molecular mass of around 37 kDa. This protein is expressed in various tissues including the pancreas heart and brain suggesting its involvement in diverse cellular functions. Within the ER RCN1 interacts with other proteins aiding in their proper folding and assembly.
Biological function summary

RCN1 plays a role in cellular calcium homeostasis and may influence processes such as protein folding and trafficking. It is not known to be a part of a larger complex but it interacts with other ER-resident proteins. Maintaining calcium levels is important within the ER where RCN1 helps buffer calcium concentrations. This function supports processes that are sensitive to calcium fluctuations such as signal transduction and stress responses.

Pathways

The role of RCN1 in cellular mechanisms connects it to important pathways like the calcium signaling pathway and the unfolded protein response pathway. In the calcium signaling pathway RCN1 aids in regulating calcium levels within the ER affecting downstream signaling events. Meanwhile in the unfolded protein response pathway RCN1 participates by ensuring the proper folding of proteins particularly during times of stress when misfolded proteins accumulate. RCN1's interaction with calumenin another calcium-binding protein highlights its involvement in these pathways.

RCN1 has associations with cardiovascular disease and some forms of cancer. In cardiovascular disease altered expression of RCN1 may impact calcium signaling and lead to dysfunction in heart cells. Similarly in certain cancers changes in RCN1 expression levels could affect cellular proliferation via calcium-regulated pathways. The interaction between RCN1 and calreticulin a multifunctional protein involved in calcium homeostasis and immune response further connects RCN1's influence on disease progression.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB210404

Anti-RCN1/RCN antibody [EPR17163-117]

RabMAb|Recombinant|KO Validated

Western blot - Anti-RCN1/RCN antibody [EPR17163-117] (AB210404)

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Cell Lines & Lysates

AB266098

Human RCN1 (RCN) knockout HEK-293T cell line

Advanced Validation

Western blot - Human RCN1 (RCN) knockout HEK-293T cell line (AB266098)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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