Human RCN2 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human RCN2 knockout HEK-293T cell lysate (AB259081)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RCN2 knockout HEK-293T cell lysate (AB259081)
Allele-3 : 2 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RCN2 knockout HEK-293T cell lysate (AB259081)
Allele-1 : 37 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein interacts with cellular mechanisms associated with calcium signaling pathways. RCN2 forms part of a larger molecular complex that influences protein processing in the endoplasmic reticulum. As a chaperone it helps in ensuring that proteins achieve their correct conformations. Research shows that RCN2 also interacts with various cell signaling molecules and contributes to the regulation of cellular stress responses.
Pathways
Involvement of RCN2 extends to the unfolded protein response (UPR) and calcium signaling pathways. In the UPR RCN2 helps maintain endoplasmic reticulum function and protein folding. RCN2 interacts with significant pathway proteins such as GRP78/BiP which is an important regulator in maintaining protein-folding homeostasis. In calcium signaling the regulation by RCN2 supports intracellular calcium balance and communication between organelles.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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