Human REPIN1 (AP4) knockout HEK-293T cell lysate
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REPIN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1.
View Alternative Names
60 kDa origin specific DNA binding protein, 60 kDa replication initiation region protein, ATT binding protein, DHFR oribeta binding protein RIP60, H_DJ0584D14.12, REPIN1, RIP60, Replication initiation region protein (60kD), Replication initiator 1, ZNF464, Zfp464, Zinc finger protein 464 (RIP60), Zinc finger proten AP4
- Sanger seq
Unknown
Sanger Sequencing - Human REPIN1 (AP4) knockout HEK-293T cell lysate (AB259082)
Allele-1 : 13 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human REPIN1 (AP4) knockout HEK-293T cell lysate (AB259082)
Allele-2 : 2 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AP4 is an essential piece of the vesicular transport machinery. It interacts with various cargo proteins sorting them into distinct vesicles for different cellular destinations. AP4 functions as part of a complex that includes other adaptins and accessory proteins each contributing specialized roles for accurate protein sorting. These interactions play significant roles in maintaining cellular homeostasis and normal neuronal function.
Pathways
AP4 is intimately involved in the endosomal-lysosomal pathway and clathrin-mediated endocytosis. These pathways are important for proper cellular protein recycling and degradation processes. AP4 also associates with other proteins like AP1 and AP2 which overlap in the sorting at different cellular locations. These connections make AP4 an essential component of the broader protein transport network within cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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