Human REXO4 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human REXO4 knockout HeLa cell lysate (AB263327)
Homozygous : 1 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RNA exonucleases like REXO4 contribute to RNA surveillance processes that maintain the quality of RNA. REXO4 might function as part of a larger complex tasked with the maturation and degradation of RNA. These activities are essential especially in the context of removing faulty RNA molecules that could cause deleterious effects if left unchecked in the cell. The precise role of REXO4 remains under investigation yet its contributions to the RNA life cycle are noteworthy.
Pathways
RNA processing and degradation involve REXO4 in essential molecular interactions. It participates in the RNA degradation pathway where it contributes to the turnover of RNA molecules ensuring cellular stability and homeostasis. This pathway often intersects with the nonsense-mediated decay pathway a critical quality control mechanism. Proteins such as UPF1 which are involved in the nonsense-mediated decay pathway might be functionally connected to REXO4 highlighting its importance in maintaining cellular RNA integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com