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AB263328

Human RHOG knockout HEK-293T cell lysate

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RHOG KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
2 Images
Western blot - Human RHOG knockout HEK-293T cell lysate (AB263328)
  • WB

Lab

Western blot - Human RHOG knockout HEK-293T cell lysate (AB263328)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg

Lane 2 : RHOG knockout HEK-293T cell lysate 20 μg

Lane 3 : Jurkat cell lysate 20 μg

Lane 4 : SH-SY5Y cell lysate 20 μg

False colour image of Western blot : Anti-RHOG antibody [1F3-RHOG] staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab254147 was shown to bind specifically to RHOG. A band was observed at 18 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in RHOG knockout cell line ab266753 (knockout cell lysate ab263328). To generate this image, wild-type and RHOG knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-RHOG antibody [1F3-RHOG] (<a href='/en-us/products/primary-antibodies/rhog-antibody-1f3-rhog-ab254147'>ab254147</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

RHOG knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human RHOG knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-rhog-knockout-hek-293t-cell-line-ab266753'>ab266753</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 18 kDa

false

Sanger Sequencing - Human RHOG knockout HEK-293T cell lysate (AB263328)
  • Sanger seq

Unknown

Sanger Sequencing - Human RHOG knockout HEK-293T cell lysate (AB263328)

Homozygous : 1 bp insertion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

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Secondary Antibodies

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Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) (AB175775)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263328-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263328-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human RHOG knockout HEK293T cell lysate", "number":"AB263328-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
RHOG
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Quality control - STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHOG also known as ras homolog gene family member G is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rho family which plays a role in various cellular functions. RHOG is highly expressed in hematopoietic cells with significant presence in macrophages and neutrophils. This protein is also found in various tissues where it assists in cell movement and cytoskeletal rearrangements.
Biological function summary

RHOG regulates cell shape and motility. It is involved in forming membrane protrusions such as filopodia and lamellipodia. RHOG can act as a part of a protein complex with other GTPases. It controls actin cytoskeleton dynamics by activating pathways needed for cell migration and chemotaxis. This protein significantly aids immune cell function during immune surveillance and inflammatory responses.

Pathways

RHOG interacts with diverse signaling cascades. It activates the Rac1 pathway influencing cellular migration and growth processes. RHOG collaborates with proteins like PI3-kinase in these pathways contributing to the regulation of cytoskeletal organization. RHOG involvement in these signaling networks ensures cells respond aptly to external cues maintaining cellular integrity and function.

RHOG is implicated in cancer and chronic inflammatory diseases. Abnormal RHOG expression or activity can lead to excessive cell movement and invasion often linked to cancer metastasis. Additionally it relates to chronic inflammatory responses where dysregulation may exacerbate disorders such as rheumatoid arthritis. In these conditions RHOG interacts with proteins like RhoA to mediate changes in cell behavior that drive disease progression.
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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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