Human RICTOR knockout A549 cell lysate
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RICTOR KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp deletion in exon 5.
View Alternative Names
AVO3, AVO3 homolog, DKFZp686B11164, KIAA1999, Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae), MGC39830, PIA, Pianissimo, RICTR_HUMAN, RPTOR independent companion of MTOR complex 2, Rapamycin-insensitive companion of mTOR, TORC2 specific protein AVO3, hAVO3, mAVO3
- WB
Lab
Western blot - Human RICTOR knockout A549 cell lysate (AB288315)
Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : RICTOR knockout A549 cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-RICTOR antibody staining at 1 μg/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab105469 was shown to bind specifically to RICTOR. A band was observed at 190 kDa in wild-type A549 cell lysates with no signal observed at this size in RICTOR knockout cell line ab277866 (knockout cell lysate ab288315). To generate this image, wild-type and RICTOR knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-RICTOR antibody (<a href='/en-us/products/primary-antibodies/rictor-antibody-ab105469'>ab105469</a>) at 1 µg/mL
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
RICTOR knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human RICTOR knockout A549 cell line (<a href='/en-us/products/cell-lines/human-rictor-knockout-a549-cell-line-ab277866'>ab277866</a>)
Lane 3:
HeLa cell lysate at 20 µg
Predicted band size: 192 kDa
Observed band size: 190 kDa
false
- WB
Lab
Western blot - Human RICTOR knockout A549 cell lysate (AB288315)
Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : RICTOR knockout A549 cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-RICTOR antibody [EPR22008] staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab219950 was shown to bind specifically to RICTOR. A band was observed at 190 kDa in wild-type A549 cell lysates with no signal observed at this size in RICTOR knockout cell line ab277866 (knockout cell lysate ab288315). To generate this image, wild-type and RICTOR knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-RICTOR antibody [EPR22008] (<a href='/en-us/products/primary-antibodies/rictor-antibody-epr22008-ab219950'>ab219950</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
RICTOR knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human RICTOR knockout A549 cell line (<a href='/en-us/products/cell-lines/human-rictor-knockout-a549-cell-line-ab277866'>ab277866</a>)
Lane 3:
HeLa cell lysate at 20 µg
Predicted band size: 192 kDa
Observed band size: 190 kDa
false
- Sanger seq
Supplier Data
Sanger Sequencing - Human RICTOR knockout A549 cell lysate (AB288315)
43 bp deletion in exon 5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein plays a significant role in cellular growth proliferation and survival. RICTOR forms part of the mTORC2 complex which is important for Akt/PKB phosphorylation. Activation of mTORC2 by RICTOR regulates actin cytoskeleton dynamics and membrane trafficking. This involvement affects the control of cell metabolism and immune responses.
Pathways
RICTOR integrates into the PI3K/Akt signaling pathway influencing cell growth and survival in response to nutrients and growth factors. RICTOR as part of mTORC2 also interacts with proteins such as Sin1 and mLST8 helping regulate PKB/Akt phosphorylation at Ser473. This interaction links RICTOR's function to the insulin signaling pathway which controls aspects of glucose metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
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Product promise
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