Human RMDN3 (PTPIP51) knockout HeLa cell lysate
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RMDN3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
Cerebral protein 10, FAM82C, FAM82C, formerly, FLJ10579, Family with sequence similarity 82 member C, Family with sequence similarity 82 member C, formerly, Family with sequence similarity 82, member A2, Microtubule Associated Protein, Protein FAM82A2, Protein FAM82C, Protein tyrosine phosphatase interacting protein 51, Regulator of microtubule dynamics 3, Regulator of microtubule dynamics protein 3, TCPTP interacting protein 51, hRMD 3
- Sanger seq
Unknown
Sanger Sequencing - Human RMDN3 (PTPIP51) knockout HeLa cell lysate (AB258637)
Allele-1 : 14 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human RMDN3 (PTPIP51) knockout HeLa cell lysate (AB258637)
Allele-2 : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PTPIP51 protein plays a role in cellular processes such as apoptosis and cellular differentiation. It is believed to associate with other proteins to form complexes which influence mitochondrial function and cellular energy metabolism. Through these interactions PTPIP51 aids in maintaining cellular homeostasis and regulating cell survival mechanisms.
Pathways
PTPIP51 participates in the regulation of the MAPK/ERK pathway and the insulin signaling pathway. It interacts with proteins such as RAF1 and GRB2 to convey signals from the cell surface to the DNA in the cell nucleus affecting cell division and proliferation. These pathways highlight the protein’s importance in controlling cellular growth and reaction to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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